Basic Vector Information
- Vector Name:
- PiggyBac-U6-sgRNA-PE2-BSD
- Antibiotic Resistance:
- Ampicillin
- Length:
- 14135 bp
- Type:
- Gene-editing, Transposition
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Blast
- Promoter:
- CMV
- 5' Primer:
- Cas9-F7
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
PiggyBac-U6-sgRNA-PE2-BSD vector Map
PiggyBac-U6-sgRNA-PE2-BSD vector Sequence
LOCUS . 14135 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter complement(1..105)
/label="AmpR promoter"
rep_origin complement(131..586)
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1365..1605
/label="U6 promoter"
/note="RNA polymerase III promoter for human U6 snRNA"
misc_RNA 3495..3570
/label="gRNA scaffold"
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter 3632..3843
/label="EF-1α core promoter"
/note="core promoter for human elongation factor EF-1α"
CDS 3899..3919
/label="SV40 NLS"
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
CDS 3917..8020
/label="Cas9(H840A)"
/note="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
CDS 10193..10213
/label="SV40 NLS"
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
CDS 10223..10279
/label="P2A"
/note="2A peptide from porcine teschovirus-1 polyprotein"
CDS 10280..10675
/label="BSD"
/note="blasticidin S deaminase"
polyA_signal 10706..10838
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter complement(12162..12192)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(12207..12228)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(12516..13104)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(13278..14135)
/label="AmpR"
/note="β-lactamase"
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