LOCUS       .                      14135 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(1..105)
                     /label="AmpR promoter"
     rep_origin      complement(131..586)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1365..1605
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        3495..3570
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     promoter        3632..3843
                     /label="EF-1α core promoter"
                     /note="core promoter for human elongation factor EF-1α"
     CDS             3899..3919
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             3917..8020
                     /label="Cas9(H840A)"
                     /note="nickase mutant of the Cas9 endonuclease from the
                     Streptococcus pyogenes Type II CRISPR/Cas system"
     CDS             10193..10213
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             10223..10279
                     /label="P2A"
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     CDS             10280..10675
                     /label="BSD"
                     /note="blasticidin S deaminase"
     polyA_signal    10706..10838
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(12162..12192)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(12207..12228)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(12516..13104)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(13278..14135)
                     /label="AmpR"
                     /note="β-lactamase"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
     1081 aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc aaagccccca
     1141 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
     1201 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
     1261 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
     1321 acacctgggg ggatacgggg aaaaggcctc caaggcctac tagtgagggc ctatttccca
     1381 tgattccttc atatttgcat atacgataca aggctgttag agagataatt agaattaatt
     1441 tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg
     1501 ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt
     1561 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac accggagacg
     1621 gttgtaaatg agcacacaaa atacacatgc taaaatatta tattctatga cctttataaa
     1681 atcaaccaaa atcttctttt taataacttt agtatcaata attagaattt ttatgttcct
     1741 ttttgcaaac ttttaataaa aatgagcaaa ataaaaaaac gctagtttta gtaactcgcg
     1801 ttgttttctt cacctttaat aatagctact ccaccacttg ttcctaagcg gtcagctcct
     1861 gcttcaatca ttttttgagc atcttcaaat gttctaactc caccagctgc tttaactaaa
     1921 gcattgtctt taacaactga cttcattagt ttaacatctt caaatgttgc acctgatttt
     1981 gaaaatcctg ttgatgtttt aacaaattct aatccagctt caacagctat ttcacaagct
     2041 ttcatgattt cttcttttgt taataaacaa ttttccataa tacatttaac aacatgtgat
     2101 ccagctgctt tttttacagc tttcatgtct tctaaaacta attcataatt tttgtctttt
     2161 aatgcaccaa tatttaatac catatcaatt tctgttgcac catctttaat tgcttcagaa
     2221 acttcgaatg cttttgtagc tgttgtgcat gcacctagag gaaaacctac aacatttgtt
     2281 attcctacat ttgtgccttt taataattct ttacaatagc ttgttcaata tgaattaaca
     2341 caaactgttg caaaatcaaa ttcaattgct tcatcacata attgtttaat ttcagctttc
     2401 gtagcatctt gttttaataa tgtgtgatct atatatttgt ttagtttcat tttttctcct
     2461 atatattcat ttttaatttt aattctttaa taatttcgtc tactttaact ttagcgtttt
     2521 gaacagattc accaacacct ataaaataaa tttttagttt aggttcagtt ccacttgggc
     2581 gaacagcaaa tcatgactta tcttctaaat aaaattttag taagtcttgt cctggcatat
     2641 tatacattcc atcgatgtag tcttcaacat taacaacttt aagtccagca atttgagtta
     2701 agggtgttgc tctcaatgat ttcattaatg gttcaatttt taatttcttt tcttctggtt
     2761 taaaattcaa gtttaaagtg aaagtgtaat atgcacccat ttctttaaat aaatcttcta
     2821 aatagtctac taatgtttta ttttgttttt tataaaatca agcagcctct gctattaata
     2881 tagaagcttg tattccatct ttatctctag ctgagtcatc aattacatat ccataacttt
     2941 cttcataagc aaaaacaaaa tttaatccgt tatcttcttc tttagcaatt tctctaccca
     3001 ttcatttaaa tccagttaaa gtttttacaa tattaactcc atatttttca tgagcgattc
     3061 tatcacccaa atcacttgtt acaaaacttg aatatagagc cggatttttt ggaatgctat
     3121 ttaagcgttt tagatttgat aattttcaat caattaaaat tggtcctgtt tgatttccat
     3181 ctaatcttac aaaatgacca tcatgtttta ttgccattcc aaatctgtca gcatctgggt
     3241 cattcataat aataatatct gcatcatgtt taataccata ttcaagcggt atttttcatg
     3301 caggatcaaa ttctggattt ggatttacaa catttttaaa tgtttcatct tcaaatgcat
     3361 gctcttcaac ctcaataacg ttatatcctg attcacgtaa tatttttggg gtaaatttag
     3421 ttcctgttcc attaactgcg ctaaaaataa tttttaaatc ttttttagct tcttgctctt
     3481 ttttgtacgt ctctgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     3541 caacttgaaa aagtggcacc gagtcggtgc ttttttgaat tcgctagcta ggtcttgaaa
     3601 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     3661 gagaagttgg ggggaggggt cggcaattga tccggtgcct agagaaggtg gcgcggggta
     3721 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     3781 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     3841 caggaccggt tctagagcca ccatgaaacg gacagccgac ggaagcgagt tcgagtcacc
     3901 aaagaagaag cggaaagtcg acaagaagta cagcatcggc ctggacatcg gcaccaactc
     3961 tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct
     4021 gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag
     4081 cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg
     4141 gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga
     4201 cagcttcttc cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg
     4261 gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat
     4321 ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta
     4381 tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa
     4441 ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct
     4501 gttcgaggaa aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag
     4561 actgagcaag agcagacggc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa
     4621 tggcctgttc ggaaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa
     4681 cttcgacctg gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct
     4741 ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa
     4801 cctgtccgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc
     4861 ccccctgagc gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct
     4921 gaaagctctc gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag
     4981 caagaacggc tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt
     5041 catcaagccc atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaacag
     5101 agaggacctg ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca
     5161 cctgggagag ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga
     5221 caaccgggaa aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct
     5281 ggccagggga aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc
     5341 ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat
     5401 gaccaacttc gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta
     5461 cgagtacttc accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag
     5521 aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac
     5581 caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt
     5641 cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca
     5701 cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat
     5761 tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg
     5821 gctgaaaacc tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag
     5881 atacaccggc tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc
     5941 cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca
     6001 gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg
     6061 ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa
     6121 gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa
     6181 gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa
     6241 gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat
     6301 cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta
     6361 cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga
     6421 ctacgatgtg gacgctatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa
     6481 ggtgctgacc agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt
     6541 cgtgaagaag atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag
     6601 aaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg
     6661 cttcatcaag agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct
     6721 ggactcccgg atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt
     6781 gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt
     6841 gcgcgagatc aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac
     6901 cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt
     6961 gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa
     7021 gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg
     7081 cgagatccgg aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga
     7141 taagggccgg gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt
     7201 gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa
     7261 cagcgataag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga
     7321 cagccccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa
     7381 gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga
     7441 gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat
     7501 catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc
     7561 ctctgccggc gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt
     7621 cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa
     7681 acagctgttt gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga
     7741 gttctccaag agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa
     7801 caagcaccgg gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct
     7861 gaccaatctg ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag
     7921 gtacaccagc accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct
     7981 gtacgagaca cggatcgacc tgtctcagct gggaggtgac tctggaggat ctagcggagg
     8041 atcctctggc agcgagacac caggaacaag cgagtcagca acaccagaga gcagtggcgg
     8101 cagcagcggc ggcagcagca ccctaaatat agaagatgag tatcggctac atgagacctc
     8161 aaaagagcca gatgtttctc tagggtccac atggctgtct gattttcctc aggcctgggc
     8221 ggaaaccggg ggcatgggac tggcagttcg ccaagctcct ctgatcatac ctctgaaagc
     8281 aacctctacc cccgtgtcca taaaacaata ccccatgtca caagaagcca gactggggat
     8341 caagccccac atacagagac tgttggacca gggaatactg gtaccctgcc agtccccctg
     8401 gaacacgccc ctgctacccg ttaagaaacc agggactaat gattataggc ctgtccagga
     8461 tctgagagaa gtcaacaagc gggtggaaga catccacccc accgtgccca acccttacaa
     8521 cctcttgagc gggctcccac cgtcccacca gtggtacact gtgcttgatt taaaggatgc
     8581 ctttttctgc ctgagactcc accccaccag tcagcctctc ttcgcctttg agtggagaga
     8641 tccagagatg ggaatctcag gacaattgac ctggaccaga ctcccacagg gtttcaaaaa
     8701 cagtcccacc ctgtttaatg aggcactgca cagagaccta gcagacttcc ggatccagca
     8761 cccagacttg atcctgctac agtacgtgga tgacttactg ctggccgcca cttctgagct
     8821 agactgccaa caaggtactc gggccctgtt acaaacccta gggaacctcg ggtatcgggc
     8881 ctcggccaag aaagcccaaa tttgccagaa acaggtcaag tatctggggt atcttctaaa
     8941 agagggtcag agatggctga ctgaggccag aaaagagact gtgatggggc agcctactcc
     9001 gaagacccct cgacaactaa gggagttcct agggaaggca ggcttctgtc gcctcttcat
     9061 ccctgggttt gcagaaatgg cagcccccct gtaccctctc accaaaccgg ggactctgtt
     9121 taattggggc ccagaccaac aaaaggccta tcaagaaatc aagcaagctc ttctaactgc
     9181 cccagccctg gggttgccag atttgactaa gccctttgaa ctctttgtcg acgagaagca
     9241 gggctacgcc aaaggtgtcc taacgcaaaa actgggacct tggcgtcggc cggtggccta
     9301 cctgtccaaa aagctagacc cagtagcagc tgggtggccc ccttgcctac ggatggtagc
     9361 agccattgcc gtactgacaa aggatgcagg caagctaacc atgggacagc cactagtcat
     9421 tctggccccc catgcagtag aggcactagt caaacaaccc cccgaccgct ggctttccaa
     9481 cgcccggatg actcactatc aggccttgct tttggacacg gaccgggtcc agttcggacc
     9541 ggtggtagcc ctgaacccgg ctacgctgct cccactgcct gaggaagggc tgcaacacaa
     9601 ctgccttgat atcctggccg aagcccacgg aacccgaccc gacctaacgg accagccgct
     9661 cccagacgcc gaccacacct ggtacacgga tggaagcagt ctcttacaag agggacagcg
     9721 taaggcggga gctgcggtga ccaccgagac cgaggtaatc tgggctaaag ccctgccagc
     9781 cgggacatcc gctcagcggg ctgaactgat agcactcacc caggccctaa agatggcaga
     9841 aggtaagaag ctaaatgttt atactgatag ccgttatgct tttgctactg cccatatcca
     9901 tggagaaata tacagaaggc gtgggtggct cacatcagaa ggcaaagaga tcaaaaataa
     9961 agacgagatc ttggccctac taaaagccct ctttctgccc aaaagactta gcataatcca
    10021 ttgtccagga catcaaaagg gacacagcgc cgaggctaga ggcaaccgga tggctgacca
    10081 agcggcccga aaggcagcca tcacagagac tccagacacc tctaccctcc tcatagaaaa
    10141 ttcatcaccc tctggcggct caaaaagaac cgccgacggc agcgaattcg agcccaagaa
    10201 gaagaggaaa gtcggatccg gcgcaacaaa cttctctctg ctgaaacaag ccggagatgt
    10261 cgaagagaat cctggaccga tggccaagcc tttgtctcaa gaagaatcca ccctcattga
    10321 aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc
    10381 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg
    10441 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac
    10501 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg
    10561 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca
    10621 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaaga
    10681 attcgatatc aagcttatcg gtgttaactt gtttattgca gcttataatg gttacaaata
    10741 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    10801 tttgtccaaa ctcatcaatg tatcttatca tgtctggaat tgactcaaat gatgtcaatt
    10861 agtctatcag aagctatctg gtctcccttc cgggggacaa gacatccctg tttaatattt
    10921 aaacagcagt gttcccaaac tgggttctta tatcccttgc tctggtcaac caggttgcag
    10981 ggtttcctgt cctcacagga acgaagtccc taaagaaaca gtggcagcca ggtttagccc
    11041 cggaattgac tggattcctt ttttagggcc cattggtatg gctttttccc cgtatccccc
    11101 caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca
    11161 ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc
    11221 ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag ggacgtaatt
    11281 acatccctgg gggctttggg ggggggctgt ccctgatatc tataacaaga aaatatatat
    11341 ataataagtt atcacgtaag tagaacatga aataacaata taattatcgt atgagttaaa
    11401 tcttaaaagt cacgtaaaag ataatcatgc gtcattttga ctcacgcggt cgttatagtt
    11461 caaaatcagt gacacttacc gcattgacaa gcacgcctca cgggagctcc aagcggcgac
    11521 tgagatgtcc taaatgcaca gcgacggatt cgcgctattt agaaagagag agcaatattt
    11581 caagaatgca tgcgtcaatt ttacgcagac tatctttcta gggttaatct agctgcatca
    11641 ggatcatatc gtcgggtctt ttttccggct cagtcatcgc ccaagctggc gctatctggg
    11701 catcggggag gaagaagccc gtgccttttc ccgcgaggtt gaagcggcat ggaaagagtt
    11761 tgccgaggat gactgctgct gcattgacgt tgagcgaaaa cgcacgttta ccatgatgat
    11821 tcgggaaggt gtggccatgc acgcctttaa cggtgaactg ttcgttcagg ccacctggga
    11881 taccagttcg tcgcggcttt tccggacaca gttccggatg gtcagcccga agcgcatcag
    11941 caacccgaac aataccggcg acagccggaa ctgccgtgcc ggtgtgcaga ttaatgacag
    12001 cggtgcggcg ctgggatatt acgtcagcga ggacgggtat cctggctgga tgccgcagaa
    12061 atggacatgg ataccccgtg agttacccgg cgggcgcgct tggcgtaatc atggtcatag
    12121 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
    12181 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
    12241 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
    12301 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
    12361 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    12421 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    12481 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    12541 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    12601 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    12661 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    12721 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    12781 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    12841 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    12901 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
    12961 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    13021 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    13081 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    13141 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    13201 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    13261 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    13321 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    13381 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    13441 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    13501 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    13561 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    13621 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    13681 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    13741 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    13801 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    13861 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    13921 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    13981 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    14041 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    14101 ggaataaggg cgacacggaa atgttgaata ctcat
//
