Basic Vector Information
- Vector Name:
- MSGV-1D3-28Z-All ITAMs-linker-EGFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7664 bp
- Type:
- Protein expression, Antibody expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Retrovirus
- 5' Primer:
- EGFP-R4
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
MSGV-1D3-28Z-All ITAMs-linker-EGFP vector Map
MSGV-1D3-28Z-All ITAMs-linker-EGFP vector Sequence
LOCUS . 7664 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
LTR 1..517
/label="5' LTR"
/note="5' long terminal repeat from murine embryonic stem
cell virus"
CDS 1020..1427
/label="gag (truncated)"
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
misc_feature 1437..1807
/label="pol region"
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
CDS 3262..3975
/label="EGFP"
/note="the original enhanced GFP (Yang et al., 1996)"
LTR 4049..4563
/label="3' LTR"
/note="3' long terminal repeat from murine embryonic stem
cell virus"
protein_bind complement(4735..4751)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
promoter complement(4759..4789)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(4804..4825)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(5113..5701)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5875..6732)
/label="AmpR"
/note="β-lactamase"
promoter complement(6733..6837)
/label="AmpR promoter"
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