LOCUS       .                       7664 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..517
                     /label="5' LTR"
                     /note="5' long terminal repeat from murine embryonic stem
                     cell virus"
     CDS             1020..1427
                     /label="gag (truncated)"
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     misc_feature    1437..1807
                     /label="pol region"
                     /note="Moloney murine leukemia virus (MMLV) pol region
                     containing the splice acceptor site"
     CDS             3262..3975
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     LTR             4049..4563
                     /label="3' LTR"
                     /note="3' long terminal repeat from murine embryonic stem
                     cell virus"
     protein_bind    complement(4735..4751)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4759..4789)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4804..4825)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5113..5701)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5875..6732)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(6733..6837)
                     /label="AmpR promoter"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttcgg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttc gggggccgtt tttgtggccc
      781 gacctgagtc ctaaaatccc gatcgtttag gactctttgg tgcacccccc ttagaggagg
      841 gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg tctgaatttt
      901 tgctttcggt ttgggaccga agccgcgccg cgcgtcttgt ctgctgcagc atcgttctgt
      961 gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa tatgggcccg ggctagcctg
     1021 ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca
     1081 accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa
     1141 cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta
     1201 agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga
     1261 cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc
     1321 ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc
     1381 cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccccc atatggccat
     1441 atgagatctt atatggggca cccccgcccc ttgtaaactt ccctgaccct gacatgacaa
     1501 gagttactaa cagcccctct ctccaagctc acttacaggc tctctactta gtccagcacg
     1561 aagtctggag acctctggcg gcagcctacc aagaacaact ggaccgaccg gtggtacctc
     1621 acccttaccg agtcggcgac acagtgtggg tccgccgaca ccagactaag aacctagaac
     1681 ctcgctggaa aggaccttac acagtcctgc tgaccacccc caccgccctc aaagtagacg
     1741 gcatcgcagc ttggatacac gccgcccacg tgaaggctgc cgaccccggg ggtggaccat
     1801 cctctagccc tcgagaagct tgccaccatg ggtgtcccta cccagctcct gggactgctc
     1861 ctgctgtgga tcaccgacgc catctgcgac atccagatga cccagagccc tgccagcctg
     1921 tctaccagcc tgggcgagac agtgaccatc cagtgtcagg ccagcgagga catctactct
     1981 ggcctggctt ggtatcagca gaagcccggc aagagccctc agctgctgat ctacggcgcc
     2041 agcgacctgc aggacggcgt gcctagcaga ttcagcggca gcggctccgg aacccagtac
     2101 agcctgaaga tcaccagcat gcagaccgag gacgagggcg tgtacttctg ccagcaaggc
     2161 ctgacctacc ctagaacctt cggaggaggc accaagctgg aactgaaggg cggaggcgga
     2221 agtggaggcg gaggatctgg cggcggaggc tctgaagtgc agctgcagca gtctggcgct
     2281 gaactggtcc ggcctggcac tagcgtgaag ctgtcctgca aggtgtccgg cgacaccatc
     2341 accttctact acatgcactt cgtgaagcag aggccaggac agggcctgga atggatcggc
     2401 agaatcgacc ctgaggacga gagcaccaag tacagcgaga agttcaagaa caaggccacc
     2461 ctgaccgccg acaccagcag caacaccgcc tacctgaagc tgtctagcct gacctccgag
     2521 gacaccgcca cctacttttg catctacggc ggctactact tcgactactg gggccagggc
     2581 gtgatggtca ccgtgtccag catcgagttc atgtaccccc ctccctacct ggacaacgag
     2641 agaagcaacg gcaccatcat ccacatcaaa gaaaagcacc tgtgccacac ccagagcagc
     2701 cccaagctgt tctgggccct ggtggtggtg gccggcgtgc tgttctgtta cggcctgctg
     2761 gtcacagtgg ccctgtgcgt gatctggacc aacagcagaa gaaacagagg cggccagagc
     2821 gactacatga acatgacccc cagaaggcca ggcctgacca gaaagcccta ccagccctac
     2881 gcccctgcca gagacttcgc cgcctacaga cccagagcca agttcagcag atccgccgag
     2941 acagccgcca acctgcagga tcccaaccag ctgtacaacg agctgaacct gggcagacgg
     3001 gaggaatacg acgtgctgga aaagaagaga gccagggacc ccgagatggg cggcaagcag
     3061 cagagaagaa gaaaccctca ggaaggcgtc tacaacgccc tgcagaaaga caagatggcc
     3121 gaggcctaca gcgagatcgg caccaagggc gagagaagaa ggggcaaggg ccacgatggc
     3181 ctgtaccagg gcctgtccac cgccaccaag gacacctacg acgccctgca catgcagacc
     3241 ctggccccca gatcaggatc tatgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     3301 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     3361 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     3421 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     3481 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     3541 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     3601 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     3661 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     3721 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     3781 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     3841 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     3901 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     3961 gacgagctgt acaagtgagt cgacggtacc gcgggcccgg gatccgataa aataaaagat
     4021 tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct
     4081 agcttaagta acgccatttt gcaaggcatg gaaaatacat aactgagaat agagaagttc
     4141 agatcaaggt taggaacaga gagacagcag aatatgggcc aaacaggata tctgtggtaa
     4201 gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tcccgccctc
     4261 agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
     4321 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
     4381 cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgatagact
     4441 gcgtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt
     4501 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt
     4561 catgggtaac agtttcttga agttggagaa caacattctg agggtaggag tcgaatatta
     4621 agtaatcctg actcaattag ccactgtttt gaatccacat actccaatac tcctgaaatc
     4681 catcgatgga gttcattatg gacagcgcag aaagagctgg ggagaattgt gaaattgtta
     4741 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     4801 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     4861 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     4921 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     4981 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     5041 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     5101 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5161 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5221 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5281 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     5341 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     5401 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     5461 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     5521 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     5581 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     5641 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     5701 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     5761 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     5821 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     5881 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     5941 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     6001 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     6061 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     6121 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     6181 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     6241 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     6301 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     6361 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     6421 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     6481 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     6541 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     6601 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     6661 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     6721 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     6781 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     6841 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     6901 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     6961 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     7021 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     7081 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     7141 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
     7201 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     7261 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     7321 ggcgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct
     7381 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
     7441 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
     7501 gccacgatgc gtccggcgta gaggcgatta gtccaatttg ttaaagacag gatatcagtg
     7561 gtccaggctc tagttttgac tcaacaatat caccagctga agcctataga gtacgagcca
     7621 tagataaaat aaaagatttt atttagtctc cagaaaaagg gggg
//
