pDC316-mCherry vector (V017745)

LOCUS       .                       4648 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_signal     190..340
                     /label="Ad5 Ψ"
                     /note="packaging signal for adenovirus serotype 5"
     promoter        462..990
                     /label="mCMV promoter"
                     /note="mouse cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             1005..1712
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             1731..1748
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    1771..1905
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(1925..1958)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(1980..1996)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2004..2020)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2028..2058)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2073..2094)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2382..2970)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3144..4001)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(4002..4106)
                     /label="AmpR promoter"
     repeat_region   4649..4750
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"