LOCUS       .                       4648 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_signal     190..340
                     /label="Ad5 Ψ"
                     /note="packaging signal for adenovirus serotype 5"
     promoter        462..990
                     /label="mCMV promoter"
                     /note="mouse cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             1005..1712
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             1731..1748
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    1771..1905
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(1925..1958)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(1980..1996)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2004..2020)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2028..2058)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2073..2094)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2382..2970)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3144..4001)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(4002..4106)
                     /label="AmpR promoter"
     repeat_region   4649..4750
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
ORIGIN
        1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt
       61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
      121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
      181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
      241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
      301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg
      361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc
      421 gggtcaaagt tggcgtttta ttattatagt cagntctaga gatatactga gtcattaggg
      481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc
      541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa
      601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa
      661 taggggtgag tcattgggtt tttccagcca tttaattaaa acgccatgta ctttcccacc
      721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat
      781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg
      841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cactcattct
      901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca
      961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattcgc caccatggtg agcaagggcg
     1021 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     1081 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     1141 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     1201 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     1261 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     1321 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     1381 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     1441 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     1501 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
     1561 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
     1621 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
     1681 actccaccgg cggcatggac gagctgtaca agcccgggag atctaagctt caccaccacc
     1741 accaccactg agagctcgtc gacttcgagc aacttgttta ttgcagctta taatggttac
     1801 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     1861 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcgtcta gcatcgaaga
     1921 tccaataact tcgtatagca tacattatac gaagttataa gtagcttggc gtaatcatgg
     1981 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     2041 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     2101 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     2161 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     2221 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     2281 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     2341 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     2401 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2461 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2521 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     2581 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2641 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2701 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2761 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2821 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2881 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     2941 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3001 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3061 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3121 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     3181 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     3241 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     3301 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     3361 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     3421 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3481 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3541 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3601 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3661 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     3721 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
     3781 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     3841 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     3901 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     3961 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     4021 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4081 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     4141 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcactct aggcaaaata
     4201 gcaccctccc gctccagaac aacatacagc gcttcacagc ggcagcctaa cagtcagcct
     4261 taccagtaaa aaagaaaacc tattaaaaaa acaccactcg acacggcacc agctcaatca
     4321 gtcacagtgt aaaaaagggc caagtgcaga gcgagtatat ataggactaa aaaatgacgt
     4381 aacggttaaa gtccacaaaa aacacccaga aaaccgcacg cgaacctacg cccagaaacg
     4441 aaagccaaaa aacccacaac ttcctcaaat cgtcacttcc gttttcccac gttacgtaac
     4501 ttcccatttt aagaaaacta caattcccaa cacatacaag ttactccgcc ctaaaaccta
     4561 cgtcacccgc cccgttccca cgccccgcgc cacgtcacaa actccacccc ctcattatca
     4621 tattggcttc aatccaaaat aaggtata
//
