Basic Vector Information
- Vector Name:
- PJQ-200SK-Rif
- Antibiotic Resistance:
- Rifampicin
- Length:
- 4996 bp
- Type:
- Gene-editing vector
- Replication origin:
- p15A ori
- Host:
- E. coli
- Selection Marker:
- SacB
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
PJQ-200SK-Rif vector Map
PJQ-200SK-Rif vector Sequence
LOCUS . 4996 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
CDS 74..1492
/label="SacB"
/note="secreted levansucrase that renders bacterial growth
sensitive to sucrose"
promoter complement(2041..2111)
/label="AmpR promoter"
primer_bind complement(2612..2628)
/label="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(2665..2683)
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(2704..2720)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(2728..2744)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
promoter complement(2752..2782)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(2797..2818)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(3211..3756)
/label="p15A ori"
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
oriT complement(4139..4248)
/label="oriT"
/note="incP origin of transfer"
promoter 4624..5069
/label="sacB promoter"
/note="sacB promoter and control region"
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