LOCUS       .                       4996 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             74..1492
                     /label="SacB"
                     /note="secreted levansucrase that renders bacterial growth
                     sensitive to sucrose"
     promoter        complement(2041..2111)
                     /label="AmpR promoter"
     primer_bind     complement(2612..2628)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(2665..2683)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2704..2720)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2728..2744)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2752..2782)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2797..2818)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3211..3756)
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     oriT            complement(4139..4248)
                     /label="oriT"
                     /note="incP origin of transfer"
     promoter        4624..5069
                     /label="sacB promoter"
                     /note="sacB promoter and control region"
ORIGIN
        1 cttgccatct tcaaacagga gggctggaag aagcagaccg ctaacacagt acataaaaaa
       61 ggagacatga acgatgaaca tcaaaaagtt tgcaaaacaa gcaacagtat taacctttac
      121 taccgcactg ctggcaggag gcgcaactca agcgtttgcg aaagaaacga accaaaagcc
      181 atataaggaa acatacggca tttcccatat tacacgccat gatatgctgc aaatccctga
      241 acagcaaaaa aatgaaaaat atcaagttcc tgaattcgat tcgtccacaa ttaaaaatat
      301 ctcttctgca aaaggcctgg acgtttggga cagctggcca ttacaaaacg ctgacggcac
      361 tgtcgcaaac tatcacggct accacatcgt ctttgcatta gccggagatc ctaaaaatgc
      421 ggatgacaca tcgatttaca tgttctatca aaaagtcggc gaaacttcta ttgacagctg
      481 gaaaaacgct ggccgcgtct ttaaagacag cgacaaattc gatgcaaatg attctatcct
      541 aaaagaccaa acacaagaat ggtcaggttc agccacattt acatctgacg gaaaaatccg
      601 tttattctac actgatttct ccggtaaaca ttacggcaaa caaacactga caactgcaca
      661 agttaacgta tcagcatcag acagctcttt gaacatcaac ggtgtagagg attataaatc
      721 aatctttgac ggtgacggaa aaacgtatca aaatgtacag cagttcatcg atgaaggcaa
      781 ctacagctca ggcgacaacc atacgctgag agatcctcac tacgtagaag ataaaggcca
      841 caaatactta gtatttgaag caaacactgg aactgaagat ggctaccaag gcgaagaatc
      901 tttatttaac aaagcatact atggcaaaag cacatcattc ttccgtcaag aaagtcaaaa
      961 acttctgcaa agcgataaaa aacgcacggc tgagttagca aacggcgctc tcggtatgat
     1021 tgagctaaac gatgattaca cactgaaaaa agtgatgaaa ccgctgattg catctaacac
     1081 agtaacagat gaaattgaac gcgcgaacgt ctttaaaatg aacggcaaat ggtacctgtt
     1141 cactgactcc cgcggatcaa aaatgacgat tgacggcatt acgtctaacg atatttacat
     1201 gcttggttat gtttctaatt ctttaactgg cccatacaag ccgctgaaca aaactggcct
     1261 tgtgttaaaa atggatcttg atcctaacga tgtaaccttt acttactcac acttcgctgt
     1321 acctcaagcg aaaggaaaca atgtcgtgat tacaagctat atgacaaaca gaggattcta
     1381 cgcagacaaa caatcaacgt ttgcgccaag cttcctgctg aacatcaaag gcaagaaaac
     1441 atctgttgtc aaagacagca tccttgaaca aggacaatta acagttaaca aataaaaacg
     1501 caaaagaaaa tgccgatggc cgcggcgttg tgacaattta ccgaacaact ccgcggccgg
     1561 gaagccgatc tcggcttgaa cgaattgcta gtcttcaatg acgtgtaaac cacggcgctt
     1621 taagtcctcc aacgaatcca acattcccct tattaattca acaggatgcc cctcccagtc
     1681 ttcaacaacg ccaacaattc tcaagggttc gcaggttcta taggactgtg ttggattacc
     1741 gggaaatctt ttgttcgtaa gattcggatc gtcttcgaac ggtcctgttg gctcaactat
     1801 gtatatgtag ccgcgaccct cgaggccaga cagtgacata gcaagctcag ctccccaaac
     1861 tgctggctcc atcaaggctg aaaagtagat gtgcttaaga atacgaccgt cctcgaaatg
     1921 agagatgaac cctgtggtta gcaagtcacc aatcgccaaa ttggctttgg ttccatgata
     1981 gaacggtcct tgcacctgct tgtaattatc atgagagatg ggaatccaat cttttaccat
     2041 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     2101 catatttgaa ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct
     2161 tgggcagcag cgaagtcgag gcatttctgt cctggctggc gaacgagcgc aaggtttcgg
     2221 tctccacgca tcgtcaggca ttggcggcca agctgttctt ctacggcaag gtgctgtgca
     2281 cggatctgcc ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc ttgccggtgg
     2341 tgctgacccc ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag catcgtttgt
     2401 tcgcccagct tctgtatgga acgggcatgc ggatcagtgg gtttgcaact gcgggtcaag
     2461 gtctggattt cgatcacggc acgatcatgc tgcgggaggg caagggctcc aaggatcggg
     2521 ccttgatgtt acccgagagc ttggcaccca cgctgcgcga gcagaattaa ttccctcgat
     2581 aagcttgata tcgaattcct gcagcccggg ggatccacta gttctagagc ggccgccacc
     2641 gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     2701 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     2761 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     2821 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagg ggaattaatt
     2881 ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca
     2941 tcagcgctag cggagtgtat actggcttac tatgttggca ctgatgaggg tgtcagtgaa
     3001 gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg tcagcagaat atgtgataca
     3061 ggatatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag
     3121 cggaaatggc ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca
     3181 gggaagtgag agggccgcgg caaagccgtt tttccatagg ctccgccccc ctgacaagca
     3241 tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca
     3301 ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg
     3361 tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt
     3421 aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg
     3481 ccttatccgg taactatcgt cttgagtcca acccggaaag acatgcaaaa gcaccactgg
     3541 cagcagccac tggtaattga tttagaggag ttagtcttga agtcatgcgc cggttaaggc
     3601 taaactgaaa ggacaagttt tggtgactgc gctcctccaa gccagttacc tcggttcaaa
     3661 gagttggtag ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca
     3721 gagcaagaga ttacgcgcag accaaaacga tctcaagaag atcatcttat taatcagata
     3781 aaatatttct agctagagtc gatcttcgcc agcagggcga ggatcgtggc atcaccgaac
     3841 cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag gcggcccagg
     3901 tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc cgtgattttg
     3961 tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc cgccgccttt
     4021 tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg gctgcccttc
     4081 ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc ggcggtagcc
     4141 ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata agggacagtg
     4201 aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg ctgacgccgt
     4261 tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa
     4321 aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta tgcagcggaa
     4381 aagcgctgct tccctgctgt tttgtggaat atctaccgac tggaaacagg caaatgcagg
     4441 aaattactga actgagggga caggcgagag acgatgccaa agagctacac cgacgagctg
     4501 gccgagtggg ttgaatcccg cgcggccaag aagcgccggc gtgatgaggc tgcggttgcg
     4561 ttcctggcgg tgagggcgga tgtcgatcga ctctagctag aggatcgatc ctttttaacc
     4621 catcacatat acctgccgtt cactattatt tagtgaaatg agatattatg atattttctg
     4681 aattgtgatt aaaaaggcaa ctttatgccc atgcaacaga aactataaaa aatacagaga
     4741 atgaaaagaa acagatagat tttttagttc tttaggcccg tagtctgcaa atccttttat
     4801 gattttctat caaacaaaag aggaaaatag accagttgca atccaaacga gagtctaata
     4861 gaatgaggtc gaaaagtaaa tcgcgcgggt ttgttactga taaagcaggc aagacctaaa
     4921 atgtgtaaag ggcaaagtgt atactttggc gtcacccctt acatatttta ggtctttttt
     4981 tattgtgcgt aactaa
//
