pAMEXlox-2 vector (V016200)

LOCUS       62056_3000        5481 bp DNA     circular SYN 21-APR-2019
DEFINITION  Cloning vector pAMEXlox-2, complete sequence.
ACCESSION   MH818810
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Lu Y.
  TITLE     Establishing a novel L-ornithine self-sufficient cell factory with 
            enhanced production of fusarinine C through metabolic engineering in
            Aureobasidium melanogenum HN6.2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Lu Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-AUG-2018) College of Marine Life, Ocean University of 
            China, 5 Yushan Road, Qingdao, Shangdong 266003, China
REFERENCE   3  (bases 1 to 5481)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-AUG-2018) College of Marine Life, Ocean University of China, 5 
            Yushan Road, Qingdao, Shangdong 266003, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2746..3129
                     /regulatory_class="promoter"
     protein_bind    complement(3178..3211)
                     /label=lox71
                     /note="Left element (LE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    complement(3222..3396)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(3440..4006)
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     regulatory      4007..4458
                     /regulatory_class="promoter"
     protein_bind    complement(4459..4492)
                     /label=lox66
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5087..5103)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"