LOCUS       62056_3000        5481 bp DNA     circular SYN 21-APR-2019
DEFINITION  Cloning vector pAMEXlox-2, complete sequence.
ACCESSION   MH818810
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Lu Y.
  TITLE     Establishing a novel L-ornithine self-sufficient cell factory with 
            enhanced production of fusarinine C through metabolic engineering in
            Aureobasidium melanogenum HN6.2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Lu Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-AUG-2018) College of Marine Life, Ocean University of 
            China, 5 Yushan Road, Qingdao, Shangdong 266003, China
REFERENCE   3  (bases 1 to 5481)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-AUG-2018) College of Marine Life, Ocean University of China, 5 
            Yushan Road, Qingdao, Shangdong 266003, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2746..3129
                     /regulatory_class="promoter"
     protein_bind    complement(3178..3211)
                     /label=lox71
                     /note="Left element (LE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    complement(3222..3396)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(3440..4006)
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     regulatory      4007..4458
                     /regulatory_class="promoter"
     protein_bind    complement(4459..4492)
                     /label=lox66
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5087..5103)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gcatgccccg ggttcactgg gcgtgtcggg gaaccaggac
     2281 ttttactttg aaaaaattag agtgttcaaa gcaggccttt gctcgaatac attagcatgg
     2341 aataatagaa taggacgtgc ggttctattt tgttggtttc taggaccgcc gtaatgatta
     2401 atagggatag tcggggggca tcagtattca attgtcagag gtgaaattct tggatttatt
     2461 gaagactaac tactgcgaaa gcatttgcca aggatgtttt cattaatcag tgaacgaaag
     2521 ttaggggatc gaagacgatc agataccgtc gtagtcttaa ccataaacta tgccgactag
     2581 ggatcgggcg atgttatcat tttgactcgc tcggcacctt acgagaaatc aaagtctttg
     2641 ggttctgggg ggagtatggt cgcaaggctg aaacttaaag aaattgacgg aagggcacca
     2701 ccaggcgtgg agcctgcggc ttaatttgac tcaacacggg gaaacggtac ccgacagttg
     2761 gctcatcatc cgttacatca tctttttttc tgctggctcc gctcggtggg ctcccaacga
     2821 agcagcaaaa aaagtgagag agaaaaacta gcttggcggg gcatccgaag ctagaccctt
     2881 tggctcgctt agtcagtgcg ccctctcaca cacactcaaa aaggccaccc ctcccgcacc
     2941 ctcttctcat caccgtcttc ataccacggt tcgtcaagca atcgtatctg gtaagctttg
     3001 acctcttgag cgggctccac tttgctattt cttggatctg ctcattcttt tcttccttcc
     3061 tctctttcta acctctcttc agaaagttca accgtacttc actcaatctt ccatacatca
     3121 ccgtcaaaca ctagtcttaa gatgcatacg cgtgagctcc tgcagggtac cgtcgactac
     3181 cgttcgtata gcatacatta tacgaagtta ttaattcggg ggatctggat tttagtactg
     3241 gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa tacaaataca
     3301 tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac cctaattccc
     3361 ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga tcgacagatc
     3421 ccggtcggca tctactttat ggacatggca tggacatgta caaagcctgt tcaccatcag
     3481 aagcagtacc gtcgtacaaa gcagtatcca aaccacacaa ggtgaaaccc attcttctgt
     3541 aagcgtggat agctggagcg ttgacgttgg taacctccaa ccacaagtga ccagcacctc
     3601 tctctctagc gaactcagta gccaaaccca tcaaagctct accgacaccg tgacctctgt
     3661 gttctggagc gacctcgatg tcttcaacgg tcaatcttct gttccaaccg gagtaagaga
     3721 caacgacgaa accagccaaa tcaccatcgt caccgtaagc gacgaaagtt ctggagtctg
     3781 gatcaccgtc ctcaccagcg tcggactcat cgtcagattc atcgtctggg aaaaccttgg
     3841 tcaatggtgg atcgactgga acttctctca aagtgaaacc gtcaccagta gcggtgactc
     3901 tgaaaacagt atcagtggtg aaggaaccgt ccaaagcctc gatagcttca gcatcacctg
     3961 gaacagaagt tctgtatctg taagcggtat cgtccaaagt ggtcattgtg actgaattgg
     4021 atgtgttaga caggttgatg tgaacgacag caacagagaa agacgcacag agttatatac
     4081 aacagagctt gatgggttgt tgtggtgtgt gaggggcttg aatctttccc caacagcttc
     4141 cgacgctcgg catgaggggc agctcaaaaa aaccgcgtca ccgacgtgct accgagatct
     4201 tcgtgctctc tcgtgtttgc ctatcaactg atatcaccaa tcatgagcca tctctatgta
     4261 caaactgccc ttggatcacg tgcttcactc gacatctgca ccatgtatcc ttcttcgaat
     4321 tgctcatctt gatcacttta ttattcaaga tgccttgcac cacacgacat gtctatcgat
     4381 cggcccgtaa cgccccgtga agttgccaga acgcctgcct gtcccgctgt ccgatggatg
     4441 catgaaggta aggatcctat aacttcgtat agcatacatt atacgaacgg tagcaagtcc
     4501 gctgggaccc tcacaacgag cacgtctttt acgtgtccac agaaagcggt atcctatacg
     4561 cctttgacgc ccgcaacgca ccttcatccc ccgagaagac acaagccatc tggcgcctgc
     4621 aagcccacga agcctccctc tcatccttct ccctcaaccc cactgtcccc ggtttcatcg
     4681 ccaccgcctc caccgaccgc acagtcaagc tctggaacat tgatcaaacc accaaccagc
     4741 cctccatggt cgtctcgcgc gacctcggcg tcggccgtgt cttttctgca aactttgctc
     4801 ccgacgaggc agtggctttc cgtctttccg ttgcgggttc aaagggtgct gttcaggtct
     4861 gggataccag caccaacaga gccgtccgtg aagcctttgc cggccgtgtc aagggcattg
     4921 acgccacaga agtcaaggag agactcgtcg ccgttgccaa cgacgaagaa gagagcgaca
     4981 gcgacgagga cgaggagaac gaggacgaag ccggtgctga cgagcgcggc tgggagagca
     5041 tgtcggagta atgtgtgttt ggcaaaagcg taccgaattc cccgggactg gccgtcgttt
     5101 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     5161 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     5221 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
     5281 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     5341 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     5401 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     5461 cgtcatcacc gaaacgcgcg a
//
