pCR-Amp-mCherry-XGPRT vector (V015707)

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LOCUS       V015707                 3671 bp    DNA     circular SYN 02-APR-2020
DEFINITION  Exported.
ACCESSION   V015707
VERSION     V015707
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Omura N, Yoshikawa T, Fujii H, Shibamura M, Inagaki T, Kato H, Egawa
            K, Harada S, Yamada S, Takeyama H, Saijo M.
  TITLE     A novel system for constructing a recombinant highly-attenuated
            vaccinia virus strain (LC16m8) expressing foreign genes and its
            application for the generation of LC16m8-based vaccines against
            herpes simplex virus 2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Yoshikawa T, Saijo M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2017) Contact:Tomoki Yoshikawa National Institute
            of Infectious Diseases, Department of Virology I; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan URL
            :https://www.niid.go.jp/niid/en/
REFERENCE   3  (bases 1 to 3671)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-SEP-2017) Contact:Tomoki Yoshikawa National Institute of
            Infectious Diseases, Department of Virology I; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan URL
            :https://www.niid.go.jp/niid/en/"
FEATURES             Location/Qualifiers
     source          1..3671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(887..1744)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1745..1815)
                     /label="AmpR promoter"
     primer_bind     1951..1967
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        1974..1992
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2072..2111
                     /label="VAC early late promoter"
                     /note="VAC early late promoter"
                     /regulatory_class="promoter"
     CDS             2112..2819
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     regulatory      2830..2869
                     /label="VAC early late promoter"
                     /note="VAC early late promoter"
                     /regulatory_class="promoter"
     CDS             2876..3331
                     /gene="gpt"
                     /label="Xanthine-guanine phosphoribosyltransferase"
                     /note="Xanthine-guanine phosphoribosyltransferase from
                     Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC).
                     Accession#: B7N8G9"
     promoter        complement(3415..3433)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3451..3467)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3475..3491)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3499..3529)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3544..3565)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."