LOCUS       V015707                 3671 bp    DNA     circular SYN 02-APR-2020
DEFINITION  Exported.
ACCESSION   V015707
VERSION     V015707
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Omura N, Yoshikawa T, Fujii H, Shibamura M, Inagaki T, Kato H, Egawa
            K, Harada S, Yamada S, Takeyama H, Saijo M.
  TITLE     A novel system for constructing a recombinant highly-attenuated
            vaccinia virus strain (LC16m8) expressing foreign genes and its
            application for the generation of LC16m8-based vaccines against
            herpes simplex virus 2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Yoshikawa T, Saijo M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2017) Contact:Tomoki Yoshikawa National Institute
            of Infectious Diseases, Department of Virology I; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan URL
            :https://www.niid.go.jp/niid/en/
REFERENCE   3  (bases 1 to 3671)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-SEP-2017) Contact:Tomoki Yoshikawa National Institute of
            Infectious Diseases, Department of Virology I; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan URL
            :https://www.niid.go.jp/niid/en/"
FEATURES             Location/Qualifiers
     source          1..3671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(887..1744)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1745..1815)
                     /label="AmpR promoter"
     primer_bind     1951..1967
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        1974..1992
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2072..2111
                     /label="VAC early late promoter"
                     /note="VAC early late promoter"
                     /regulatory_class="promoter"
     CDS             2112..2819
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     regulatory      2830..2869
                     /label="VAC early late promoter"
                     /note="VAC early late promoter"
                     /regulatory_class="promoter"
     CDS             2876..3331
                     /gene="gpt"
                     /label="Xanthine-guanine phosphoribosyltransferase"
                     /note="Xanthine-guanine phosphoribosyltransferase from
                     Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC).
                     Accession#: B7N8G9"
     promoter        complement(3415..3433)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3451..3467)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3475..3491)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3499..3529)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3544..3565)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaagccca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaccaa tgcttaatca
      901 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
      961 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     1021 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     1081 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     1141 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     1201 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     1261 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     1321 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     1381 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     1441 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     1501 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     1561 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     1621 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     1681 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     1741 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     1801 gatacatatt tgaacgtacg tataggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg
     1981 actcactata gggcgaattg ggccctctag atgcatgctc gagcggccgc cagtgtgatg
     2041 gatatctgca gaattcgccc ttgggaccgg taaaaattga aattttattt tttttttttg
     2101 gaatataaat aatggtgagc aagggcgagg aggacaacat ggccatcatc aaggagttca
     2161 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     2221 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg
     2281 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     2341 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     2401 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     2461 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     2521 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     2581 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     2641 actacgacgc cgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     2701 cctacaacgt caacatcaag ctggacatca cctcccacaa cgaggactac accatcgtgg
     2761 aacagtacga gcgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
     2821 aatttttata aaaattgaaa ttttattttt tttttttgga atataaatag ccaccatgag
     2881 cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg
     2941 actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctggtacc
     3001 gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag
     3061 ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga
     3121 aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga
     3181 aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt
     3241 tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg
     3301 cgtcgtattc gtcccgccaa tctccggtcg ctaattttta tgaattccag cacactggcg
     3361 gccgttacta gtggatccga gctcggtacc aagcttgatg catagcttga gtattctata
     3421 gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     3481 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     3541 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     3601 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     3661 tattgggcgc t
//
