Basic Vector Information
- Vector Name:
- pMIND-RC1973
- Antibiotic Resistance:
- Kanamycin
- Length:
- 7225 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- E. coli
- Selection Marker:
- Hyg
- Promoter:
- TetRO
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pMIND-RC1973 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMIND-RC1973 vector Sequence
LOCUS V014314 7225 bp DNA circular SYN 01-JAN-1980 DEFINITION Exported. ACCESSION V014314 VERSION V014314 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 7225) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..7225 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 19..35 /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" CDS 1355..2350 /gene="hyg" /label="Hygromycin-B 7''-O-kinase" /note="Hygromycin-B 7''-O-kinase from Streptomyces hygroscopicus. Accession#: P09979" primer_bind complement(2363..2379) /label="M13 fwd" /note="common sequencing primer, one of multiple similar variants" CDS 5192..5983 /label="NeoR/KanR" /note="aminoglycoside phosphotransferase" rep_origin 6270..6858 /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 7146..7167 /label="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." promoter 7182..7212 /label="lac promoter" /note="promoter for the E. coli lac operon"
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