LOCUS       V014314                 7225 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014314
VERSION     V014314
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..35
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1355..2350
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     primer_bind     complement(2363..2379)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             5192..5983
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      6270..6858
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    7146..7167
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7182..7212
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 gcggataaca atttcacaca ggaaacagct atgacatgat tacgaattag cccgcctaat
       61 gagcgggctt ttttttgaat tagcccgcct aatgagcggg cttttttttg aattcgagct
      121 cggtacctct agatcacgat tcgctcgagg tcaatgacgc tgtgcgggag tgagagaatc
      181 cgtcgatcag tgctgtcagg ccgaaatcga attcccggtc gcgttcagga acgctcgcat
      241 tgccgacctc tgcggtttgc tcctccagta cgacaccgac ggtgtatcgg ctgaccgcga
      301 tgagcatgct gatggctgtc tgttcatcga atccctgaga cacgagaagc ccgacttgct
      361 gaccggaagc ctcagctcct tcgtcggtgt cgtcgccgga tggtctgcgg tggtgctcag
      421 cgtggagacg cgcaccgtca cggaccgcgt gtagtgcgtg gcggaaactt cttgcgtttc
      481 gcaagagaaa tgcgtcccat ttctcgtcgg actcggggaa ggaagcgtga tgctctcggt
      541 caagcacgtc gctcgccagc gctgcgagga gttcggcctt cgtgcggaag tgccagtaga
      601 ggccgggctg ctgtacctgt aagtgagccg ccagcgcgcg agtggtgaag ccatcgagcc
      661 cagtctcgtc gagcacctgc cgggccccga gcaacacgga cgtgcggtcg agacgcttcc
      721 ggtggtgagt catagttgca ctttatcatc gataactcta tcttagataa agtgactgct
      781 cgctactctc atctgactgc tcgctactct catcgtggaa tcctgacagg atcccttaag
      841 aactgcagtc catatggata tcatgagctg gtcgcgggtg atcgcctacg ggctgctgcc
      901 cgggctggcg ttggcgctga cgtgtggcgc gggcttgctg aaatggcagg acggcgccgt
      961 ccgcgacgcc gcggttgccc gtgcggaatc cgtgcgggcc gcgaccgacg gcaccaccgc
     1021 gctgctgtct taccggcccg acaccgtgca gcatgacctc gagagcgcgc gaagcaggct
     1081 cacgggcacg ttcctcgacg cctacacaca gctgacccac gacgtggtga tccccggcgc
     1141 acagcagaag cagatctcgg ccgtggccac cgtcgcggcc gcggcgtcgg tgtcgacttc
     1201 cgccgaccgc gccgtcgtcc tgctgttcgt aaaccagacc atcaccgtcg gcaaggacgc
     1261 gccgaccacc gccgcttcca gcgttcgggt gaccctcgac aacatcaacg ggcgttggct
     1321 gatctcgcaa ttcgaaccga tcactagtgc atgcgtgaca caagaatccc tgttacttct
     1381 cgaccgtatt gattcggatg attcctacgc gagcctgcgg aacgaccagg aattctggga
     1441 gccgctggcc cgccgagccc tggaggagct cgggctgccg gtgccgccgg tgctgcgggt
     1501 gcccggcgag agcaccaacc ccgtactggt cggcgagccc ggcccggtga tcaagctgtt
     1561 cggcgagcac tggtgcggtc cggagagcct cgcgtcggag tcggaggcgt acgcggtcct
     1621 ggcggacgcc ccggtgccgg tgccccgcct cctcggccgc ggcgagctgc ggcccggcac
     1681 cggagcctgg ccgtggccct acctggtgat gagccggatg accggcacca cctggcggtc
     1741 cgcgatggac ggcacgaccg accggaacgc gctgctcgcc ctggcccgcg aactcggccg
     1801 ggtgctcggc cggctgcaca gggtgccgct gaccgggaac accgtgctca ccccccattc
     1861 cgaggtcttc ccggaactgc tgcgggaacg ccgcgcggcg accgtcgagg accaccgcgg
     1921 gtggggctac ctctcgcccc ggctgctgga ccgcctggag gactggctgc cggacgtgga
     1981 cacgctgctg gccggccgcg aaccccggtt cgtccacggc gacctgcacg ggaccaacat
     2041 cttcgtggac ctggccgcga ccgaggtcac cgggatcgtc gacttcaccg acgtctatgc
     2101 gggagactcc cgctacagcc tggtgcaact gcatctcaac gccttccggg gcgaccgcga
     2161 gatcctggcc gcgctgctcg acggggcgca gtggaagcgg accgaggact tcgcccgcga
     2221 actgctcgcc ttcaccttcc tgcacgactt cgaggtgttc gaggagaccc cgctggatct
     2281 ctccggcttc accgatccgg aggaactggc gcagttcctc tgggggccgc cagacaccgc
     2341 ccccggcgcc taaaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     2401 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     2461 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     2521 gataagctag cccaaccagc ccgcgaacgg ctggcagcca gcgtaaggcg cggctcatcg
     2581 ggcggcgttc gccacgatgt cctgcacttc gagccaagcc tcgaacacct gctggtgtgc
     2641 acgactcacc cggttgttga caccgcgcgc ggccgtgcgg gctcggtggg gcggctctgt
     2701 cgcccttgcc agcgtgagta gcgcgtacct cacctcgccc aacaggtcgc acacagccga
     2761 ttcgtacgcc ataaagccag gtgagcccac cagctccgta agttcgggcg ctgtgtggct
     2821 cgtacccgcg cattcaggcg gcagggggtc taacgggtct aaggcggcgt gtacggccgc
     2881 cacagcggct ctcagcggcc cggaaacgtc cttgaaacga cgtatgtgtt cctcctggtt
     2941 ggtacaggtg gttgggggtg ctcggctgtc gctggtgttc caccaccagg gctcgacggg
     3001 agagcggggg agtgtgcagt tgtggggtgg cccctcagcg aaatatctga cttggagctc
     3061 gtgtcggacc atacaccggt gattaatcgt ggtctactac caagcgtgag ccacgtcgcc
     3121 gacgaatttg agcagctctg gctgccgtac tggccgctgg caagcgacga tctgctcgag
     3181 gggatctacc gccaaagccg cgcgtcggcc ctaggccgcc ggtacatcga ggcgaaccca
     3241 acagcgctgg caaacctgct ggtcgtggac gtagaccatc cagacgcagc gctccgagcg
     3301 ctcagcgccc gggggtccca tccgctgccc aacgcgatcg tgggcaatcg cgccaacggc
     3361 cacgcacacg cagtgtgggc actcaacgcc cctgttccac gcaccgaata cgcgcggcgt
     3421 aagccgctcg catacatggc ggcgtgcgcc gaaggccttc ggcgcgccgt cgacggcgac
     3481 cgcagttact caggcctcat gaccaaaaac cccggccaca tcgcctggga aacggaatgg
     3541 ctccactcag atctctacac actcagccac atcgaggccg agctcggcgc gaacatgcca
     3601 ccgccgcgct ggcgccagca gaccacgtac aaagcggctc cgacgccgct agggcggaat
     3661 tgcgcactgt tcgattccgt caggttgtgg gcctatcgtc ccgccctcat gcggatctac
     3721 ctgccgaccc ggaacgtgga cggactcggc cgcgcgatct atgccgagtg ccacgtccga
     3781 aacgccgagt tcccgtgcaa cgacgtgtgt cccggaccgc taccggacag cgaggtccgc
     3841 gccatcgcca acagcatttg gcgttggatc acaaccaagt cgcgcatttg ggcggacggg
     3901 atcgtggtct acgaggccac actcagtgcg cgccagtcgg ccatctcgcg gaagggcgca
     3961 gcagcgcgca cggcggcgag cacagttgcg cggcgcgcaa agtccgcgtc agccatggag
     4021 gcattgctat gagcgacggc tacagcgacg gctacaaccg gcagccgact gtccgcaaaa
     4081 agcggcgcgt gaccgccgcc gaaggcgctc gaatcaccgg actatccgaa cgccacgtcg
     4141 tccggctcgt ggcgcaggaa cgcagcgagt ggctcgccga gcaggctgca cgccgcgaac
     4201 gcatccgcgc ctatcacgac gacgagggcc actcttggcc gcaaacggcc aaacatttcg
     4261 ggctgcatct ggacaccgtt aagcgactcg gctatcgggc gaggaaagag cgtgcggcag
     4321 aacaggaagc ggctcaaaag gcccacaacg aagccgacaa tccaccgctg ttctaacgca
     4381 attggggagc gggtgtcgcg ggggttccgt ggggggttcc gttgcaacgg gtcggacagg
     4441 taaaagtcct ggtagacgct agttttctgg tttgggccat gcctgtctcg ttgcgtgttt
     4501 cgttgcgccc gttttgaata ccagccagac gagacggggt tctacgaatc ttggtcgata
     4561 ccaagccatt tccgctgaat atcggggagc tcaccgccag aatcggtggt tgtggtgatg
     4621 tacgtggcga actccgttgt agtgcctgtg gtggcatccg tggccactct cgttgcacgg
     4681 ttcgttgtgc cgttacaggc cccgttgaca gctcaccgaa cgtagttaaa acatgctggt
     4741 caaactaggt ttaccaacga tacgagtcag ctcatctagg gccagttcta ggcgttgttc
     4801 gttgcgcggt tcgttgcgca tgtttcgtgt ggttgctagc ttcacgctgc cgcaagcact
     4861 cagggcgcaa gggctgctaa aggaagcgga acacgtagaa agccagtccg cagaaacggt
     4921 gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa
     4981 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat
     5041 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct
     5101 gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg gatcaagatc
     5161 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     5221 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     5281 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     5341 gaccgacctg tccggtgccc tgaatgaact ccaagacgag gcagcgcggc tatcgtggct
     5401 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     5461 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     5521 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     5581 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     5641 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     5701 gttcgccagg ctcaaggcgc ggatgcccga cggcgaggat ctcgtcgtga cccatggcga
     5761 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     5821 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     5881 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     5941 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     6001 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     6061 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     6121 cagcgcgggg atctcatgct ggagttcttc gcccacccca aaaggatcta ggtgaagatc
     6181 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6241 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6301 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6361 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6421 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6481 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6541 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6601 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6661 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6721 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6781 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6841 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6901 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6961 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7021 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7081 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7141 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7201 gctcgtatgt tgtgtggaat tgtga
//
