pMHE7Tc vector (V004732)

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LOCUS       40924_30855        6014 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range expression vector pMHE7Tc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Fodor BD.
  TITLE     Modular broad-host-range expression vectors for the purification of 
            proteins and protein complexes and their application
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Fodor BD.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2003) Biophysics, BRC Hungarian Academy of 
            Sciences, Temesvari, Szeged, Csongrad 6701, Hungary
REFERENCE   3  (bases 1 to 6014)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6014)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-MAY-2003) Biophysics, BRC Hungarian Academy of Sciences, 
            Temesvari, Szeged, Csongrad 6701, Hungary"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        189..207
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    208..232
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             247..269
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    276..278
                     /label=start codon of fusion protein
                     /note="start codon of fusion protein"
     CDS             282..305
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             318..341
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             342..353
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     terminator      466..513
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1147..1806)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(1807..2576)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     misc_feature    2800..3793
                     /label=RP4 mob, oriT
                     /note="RP4 mob, oriT"
     regulatory      complement(3883..3998)
                     /label=T4 terminator
                     /note="T4 terminator"
                     /regulatory_class="terminator"
     CDS             complement(4307..5494)
                     /label=TcR
                     /note="tetracycline efflux protein"
     regulatory      5879..5994
                     /label=T4 terminator
                     /note="T4 terminator"
                     /regulatory_class="terminator"