LOCUS       40924_30855        6014 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range expression vector pMHE7Tc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Fodor BD.
  TITLE     Modular broad-host-range expression vectors for the purification of 
            proteins and protein complexes and their application
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Fodor BD.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2003) Biophysics, BRC Hungarian Academy of 
            Sciences, Temesvari, Szeged, Csongrad 6701, Hungary
REFERENCE   3  (bases 1 to 6014)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6014)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-MAY-2003) Biophysics, BRC Hungarian Academy of Sciences, 
            Temesvari, Szeged, Csongrad 6701, Hungary"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        189..207
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    208..232
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             247..269
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    276..278
                     /label=start codon of fusion protein
                     /note="start codon of fusion protein"
     CDS             282..305
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             318..341
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             342..353
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     terminator      466..513
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1147..1806)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(1807..2576)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     misc_feature    2800..3793
                     /label=RP4 mob, oriT
                     /note="RP4 mob, oriT"
     regulatory      complement(3883..3998)
                     /label=T4 terminator
                     /note="T4 terminator"
                     /regulatory_class="terminator"
     CDS             complement(4307..5494)
                     /label=TcR
                     /note="tetracycline efflux protein"
     regulatory      5879..5994
                     /label=T4 terminator
                     /note="T4 terminator"
                     /regulatory_class="terminator"
ORIGIN
        1 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
       61 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
      121 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc
      181 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
      241 aataattttg tttaacttta agaaggagat ataccatgga cgactacaag gacgacgacg
      301 acaagctcga gggcaactgg agccatccgc agttcgagaa gatcgaggga aggcctgtcg
      361 acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccggc tgctaacaaa
      421 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
      481 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatcg
      541 cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt taatattttg
      601 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgactgc
      661 gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct
      721 ggtgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
      781 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
      841 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
      901 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
      961 ccagagcctg ataaaaacgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc
     1021 gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg
     1081 gcgaggcggc tacagccgat agtctggaac agcgcactta cgggttgctg cgcaacccaa
     1141 gtgctaccgg cgcggcagcg tgacccgtgt cggcggctcc aacggctcgc catcgtccag
     1201 aaaacacggc tcatcgggca tcggcaggcg ctgctgcccg cgccgttccc attcctccgt
     1261 ttcggtcaag gctggcaggt ctggttccat gcccggaatg ccgggctggc tgggcggctc
     1321 ctcgccgggg ccggtcggta gttgctgctc gcccggatac agggtcggga tgcggcgcag
     1381 gtcgccatgc cccaacagcg attcgtcctg gtcgtcgtga tcaaccacca cggcggcact
     1441 gaacaccgac aggcgcaact ggtcgcgggg ctggccccac gccacgcggt cattgaccac
     1501 gtaggccgac acggtgccgg ggccgttgag cttcacgacg gagatccagc gctcggccac
     1561 caagtccttg actgcgtatt ggaccgtccg caaagaacgt ccgatgagct tggaaagtgt
     1621 cttctggctg accaccacgg cgttctggtg gcccatctgc gccacgaggt gatgcagcag
     1681 cattgccgcc gtgggtttcc tcgcaataag cccggcccac gcctcatgcg ctttgcgttc
     1741 cgtttgcacc cagtgaccgg gcttgttctt ggcttgaatg ccgatttctc tggactgcgt
     1801 ggccatgctt atctccatgc ggtagggtgc cgcacggttg cggcaccatg cgcaatcagc
     1861 tgcaactttt cggcagcgcg acaacaatta tgcgttgcgt aaaagtggca gtcaattaca
     1921 gattttcttt aacctacgca atgagctatt gcggggggtg ccgcaatgag ctgttgcgta
     1981 cccccctttt ttaagttgtt gatttttaag tctttcgcat ttcgccctat atctagttct
     2041 ttggtgccca aagaagggca cccctgcggg gttcccccac gccttcggcg cggctccccc
     2101 tccggcaaaa agtggcccct ccggggcttg ttgatcgact gcgcggcctt cggccttgcc
     2161 caaggtggcg ctgccccctt ggaacccccg cactcgccgc cgtgaggctc ggggggcagg
     2221 cgggcgggct tcgccttcga ctgcccccac tcgcataggc ttgggtcgtt ccaggcgcgt
     2281 caaggccaag ccgctgcgcg gtcgctgcgc gagccttgac ccgccttcca cttggtgtcc
     2341 aaccggcaag cgaagcgcgc aggccgcagg ccggaggctt ttccccagag aaaattaaaa
     2401 aaattgatgg ggcaaggccg caggccgcgc agttggagcc ggtgggtatg tggtcgaagg
     2461 ctgggtagcc ggtgggcaat ccctgtggtc aagctcgtgg gcaggcgcag cctgtccatc
     2521 agcttgtcca gcagggttgt ccacgggccg agcgaagcga gccagccggt ggccgctcgc
     2581 ggccatcgtc cacatatcca cgggctggca agggagcgca gcgaccgcgc agggcgaagc
     2641 ccggagagca agcccgtagg gcgccgcagc cgccgtaggc ggtcacgact ttgcgaagca
     2701 aagtctagtg agtatactca agcattgagt ggcccgccgg aggcaccgcc ttgcgctgcc
     2761 cccgtcgagc cggttggaca ccaaaaggga ggggcaggca tggcggcata cgcgatcatg
     2821 cgatgcaaga agctggcgaa aatgggcaac gtggcggcca gtctcaagca cgcctaccgc
     2881 gagcgcgaga cgcccaacgc tgacgccagc aggacgccag agaacgagca ctgggcggcc
     2941 agcagcaccg atgaagcgat gggccgactg cgcgagttgc tgccagagaa gcggcgcaag
     3001 gacgctgtgt tggcggtcga gtacgtcatg acggccagcc cggaatggtg gaagtcggcc
     3061 agccaagaac agcaggcggc gttcttcgag aaggcgcaca agtggctggc ggacaagtac
     3121 ggggcggatc gcatcgtgac ggccagcatc caccgtgacg aaaccagccc gcacatgacc
     3181 gcgttcgtgg tgccgctgac gcaggacggc aggctgtcgg ccaaggagtt catcggcaac
     3241 aaagcgcaga tgacccgcga ccagaccacg tttgcggccg ctgtggccga tctagggctg
     3301 caacggggca tcgagggcag caaggcacgt cacacgcgca ttcaggcgtt ctacgaggcc
     3361 ctggagcggc caccagtggg ccacgtcacc atcagcccgc aagcggtcga gccacgcgcc
     3421 tatgcaccgc agggattggc cgaaaagctg ggaatctcaa agcgcgttga gacgccggaa
     3481 gccgtggccg accggctgac aaaagcggtt cggcaggggt atgagcctgc cctacaggcc
     3541 gccgcaggag cgcgtgagat gcgcaagaag gccgatcaag cccaagagac ggcccgagac
     3601 cttcgggagc gcctgaagcc cgttctggac gccctggggc cgttgaatcg ggatatgcag
     3661 gccaaggccg ccgcgatcat caaggccgtg ggcgaaaagc tgctgacgga acagcgggaa
     3721 gtccagcgcc agaaacaggc ccagcgccag caggaacgcg ggcgcgcaca tttccccgaa
     3781 aagtgccacc tggcggcgtt gtgacaattt accgaacaac tccgcggccg ggaagccgat
     3841 ctcggcttga acgaattgtt aggtggcggt acttgggtcg atagctttat gcttgtaaac
     3901 cgttttgtga aaaaattttt aaaataaaaa aggggacctc tagggtcccc aattaattag
     3961 taatataatc tattaaaggt cattcaaaag gtcatccacc ggatcaattc ccctgctcgc
     4021 gcaggctggg tgccaagctc tcgggtaaca tcaaggcccg atccttggag cccttgccct
     4081 cccgcacgat gatcgtgccg tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct
     4141 cactgatccg ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg
     4201 ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt
     4261 cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc
     4321 ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa
     4381 tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg
     4441 gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat
     4501 ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg
     4561 aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga
     4621 cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt
     4681 tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa
     4741 gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga
     4801 gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga
     4861 cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca
     4921 tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
     4981 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
     5041 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
     5101 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
     5161 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatccacagg acgggtgtgg
     5221 tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc
     5281 ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat
     5341 atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca
     5401 agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc
     5461 cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat
     5521 ttaactgtga taaactaccg cattaaagct cgcgcagatc agttggaaga atttgtccac
     5581 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc
     5641 cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct
     5701 cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc
     5761 tacacaaatt gggagatata tcatgaaagg ctggcttttt cttgttatcg caatagttgg
     5821 cgaagtaatc gcaacatccg cattaaaatc tagcgagggc tttactaagc tgatccggtg
     5881 gatgaccttt tgaatgacct ttaatagatt atattactaa ttaattgggg accctagagg
     5941 tccccttttt tattttaaaa attttttcac aaaacggttt acaagcataa agctcaagga
     6001 gatggcgccc aaca
//
