pMAD2 vector (V004847)

LOCUS       V004847                 9670 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004847
VERSION     V004847
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9670)
  AUTHORS   Bonnin RA, Le Lam TN, Bohn C, Bouloc P.
  TITLE     Deciphering RNase III essentiality in Staphylococcus aureus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9670)
  AUTHORS   Bonnin RA, Le Lam TN, Bohn C, Bouloc P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2015) Institut de Biologie Integrative de la
            Cellule, Faculte Paris Sud, Batiment 400, Orsay 91400, France
REFERENCE   3  (bases 1 to 9670)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9670)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-JUL-2015) Institut de Biologie Integrative de la Cellule,
            Faculte Paris Sud, Batiment 400, Orsay 91400, France"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /label="AmpR promoter"
     CDS             complement(303..1034)
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     CDS             1446..2654
                     /gene="pre"
                     /label="Plasmid recombination enzyme type 1"
                     /note="Plasmid recombination enzyme type 1 from
                     Staphylococcus aureus. Accession#: P03857"
     CDS             complement(4179..6194)
                     /gene="bgaB"
                     /label="Beta-galactosidase bgaB"
                     /note="Beta-galactosidase bgaB from Geobacillus
                     kaustophilus. Accession#: P19668"
     CDS             7432..7620
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    7725..7865
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8051..8639)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8813..9670)
                     /label="AmpR"
                     /note="beta-lactamase"