LOCUS       V004847                 9670 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004847
VERSION     V004847
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9670)
  AUTHORS   Bonnin RA, Le Lam TN, Bohn C, Bouloc P.
  TITLE     Deciphering RNase III essentiality in Staphylococcus aureus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9670)
  AUTHORS   Bonnin RA, Le Lam TN, Bohn C, Bouloc P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2015) Institut de Biologie Integrative de la
            Cellule, Faculte Paris Sud, Batiment 400, Orsay 91400, France
REFERENCE   3  (bases 1 to 9670)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9670)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-JUL-2015) Institut de Biologie Integrative de la Cellule,
            Faculte Paris Sud, Batiment 400, Orsay 91400, France"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /label="AmpR promoter"
     CDS             complement(303..1034)
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     CDS             1446..2654
                     /gene="pre"
                     /label="Plasmid recombination enzyme type 1"
                     /note="Plasmid recombination enzyme type 1 from
                     Staphylococcus aureus. Accession#: P03857"
     CDS             complement(4179..6194)
                     /gene="bgaB"
                     /label="Beta-galactosidase bgaB"
                     /note="Beta-galactosidase bgaB from Geobacillus
                     kaustophilus. Accession#: P19668"
     CDS             7432..7620
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    7725..7865
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8051..8639)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8813..9670)
                     /label="AmpR"
                     /note="beta-lactamase"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
      181 tatcacgagg ccctttcgtc ttcaagaatt aattctcatg tttgacagct tatcatcgat
      241 tcacaaaaaa taggcacacg aaaaacaagt taagggatgc agtttatgca tcccttaact
      301 tacttattaa ataatttata gctattgaaa agagataaga attgttcaaa gctaatattg
      361 tttaaatcgt caattcctgc atgttttaag gaattgttaa attgattttt tgtaaatatt
      421 ttcttgtatt ctttgttaac ccatttcata acgaaataat tatacttttg tttatctttg
      481 tgtgatattc ttgatttttt tctacttaat ctgataagtg agctattcac tttaggttta
      541 ggatgaaaat attctcttgg aaccatactt aatatagaaa tatcaacttc tgccattaaa
      601 agtaatgcca atgagcgttt tgtatttaat aatcttttag caaacccgta ttccacgatt
      661 aaataaatct cattagctat actatcaaaa acaattttgc gtattatatc cgtacttatg
      721 ttataaggta tattaccata tattttatag gattggtttt taggaaattt aaactgcaat
      781 atatccttgt ttaaaacttg gaaattatcg tgatcaacaa gtttattttc tgtagttttg
      841 cataatttat ggtctatttc aatggcagtt acgaaattac acctctttac taattcaagg
      901 gtaaaatggc cttttcctga gccgatttca aagatattat catgttcatt taatcttata
      961 tttgtcatta ttttatctat attatgtttt gaagtaataa agttttgact gtgttttata
     1021 tttttctcgt tcattataac cctctttaat ttggttatat gaattttgct tattaacgat
     1081 tcattataac cacttatttt ttgtttggtt gataatgaac tgtgctgatt acaaaaatac
     1141 taaaaatgcc catatttttt cctccttata aaattagtat aattatagca cgagctctga
     1201 taaatatgaa catgatgagt gatcgttaaa tttatactgc aatcggatgc gattattgaa
     1261 taaaagatat gagagattta tctaatttct tttttcttgt aaaaaaagaa agttcttaaa
     1321 ggttttatag ttttggtcgt agagcacacg gtttaacgac ttaattacga agtaaataag
     1381 tctagtgtgt tagactttat gaaatctata tacgtttata tatatttatt atccggaggt
     1441 gtagcatgtc tcattcaatt ttgagggttg ccagagttaa aggatcaagt aatacaaacg
     1501 ggatacaaag acataatcaa agagagaata aaaactataa taataaagac ataaatcatg
     1561 aggaaacata taaaaattat gatttgatta acgcacaaaa tataaagtat aaagataaaa
     1621 ttgatgaaac gattgatgag aattattcag ggaaacgtaa aattcggtca gatgcaattc
     1681 gacatgtgga cggactggtt acaagtgata aagatttctt tgatgattta agcggagaag
     1741 aaatagaacg attttttaaa gatagcttgg agtttctaga aaatgaatac ggtaaggaaa
     1801 atatgctgta tgcgactgtc catctggatg aaagagtccc acatatgcac tttggttttg
     1861 tccctttaac agaggacggg agattgtctg caaaagaaca gttaggcaac aagaaagact
     1921 ttactcaatt acaagataga tttaatgagt atgtgaatga gaaaggttat gaacttgaaa
     1981 gaggcacgtc caaagaggtt acagaacgag aacataaagc gatggatcag tacaagaaag
     2041 atactgtatt tcataaacag gaactgcaag aagttaagga tgagttacag aaggcaaata
     2101 agcagttaca gagtggaata gagcatatga ggtctacgaa accctttgat tatgaaaatg
     2161 agcgtacagg tttgttctct ggacgtgaag agactggtag aaagatatta actgctgatg
     2221 aatttgaacg cctgcaagaa acaatctctt ctgcagaacg gattgttgat gattacgaaa
     2281 atattaagag cacagactat tacacagaaa atcaagaatt aaaaaaacgt agagagagtt
     2341 tgaaagaagt agtgaataca tggaaagagg ggtatcacga aaaaagtaaa gaggttaata
     2401 aattaaagcg agagaatgat agtttgaatg agcagttgaa tgtatcagag aaatttcaag
     2461 atagtacagt gactttatat cgtgctgcga gggcgaattt ccctgggttt gagaaagggt
     2521 ttaataggct taaagagaaa ttctttaatg attccaaatt cgagcgtgtg ggacagttta
     2581 tggatgttgt acaggataat gtccagaagg tcgatagaaa gcgtgagaaa cagcgtacag
     2641 acgatttaga gatgtagagg tacttttatg ccgagaaaac tttttgcgtg tgacagtcct
     2701 taaaatatac ttagagcgta agcgaaagta gtagcgacag ctattaactt tcggttgcaa
     2761 agctctagga tttttaatgg acgcagcgca tcacacgcaa aaaggaaatt ggaataaatg
     2821 cgaaatttga gatgttaatt aaagaccttt ttgaggtctt tttttcttag atttttgggg
     2881 ttatttaggg gagaaaacat aggggggtac tacgacctcc cccctaggtg tccattgtcc
     2941 attgtccaaa caaataaata aatattgggt ttttaatgtt aaaaggttgt tttttatgtt
     3001 aaagtgaaaa aaacagatgt tgggaggtac agtgatggtt gtagatagaa aagaagagaa
     3061 aaaagttgct gttactttaa gacttacaac agaagaaaat gagatattaa atagaatcaa
     3121 agaaaaatat aatattagca aatcagatgc aaccggtatt ctaataaaaa aatatgcaaa
     3181 ggaggaatac ggtgcatttt aaacaaaaaa agatagacag cactggcatg ctgcctatct
     3241 atgactaaat tttgttaaat gtattagcac cgttattata tcatgagcga aaatgtaata
     3301 aaagaaactg aaaacaagaa aaattcaaga ggacgtaatt ggacatttgt tttatatcca
     3361 gaatcagcaa aagccgagtg gttagagtat ttaaaagagt tacacattca atttgtagtg
     3421 tctccattac atgataggga tactgataca gaagatagga tgaaaaaaga gcattatcat
     3481 attctagtga tgtatgaggg taataaatct tatgaacaga taaaaataat tacagaagaa
     3541 ttgaatgcga ctattccgca gattgcagga agtgtgaaag gtcttgtgag atatatgctt
     3601 cacatggacg atcctaataa atttaaatat caaaaagaag atatgatagt ttatggcggt
     3661 gtagatgttg atgaattatt aaagaaaaca acaacagata gatataaatt aattaaagaa
     3721 atgattgagt ttattgatga acaaggaatc gtagaattta agagtttaat ggattatgca
     3781 atgaagttta aatttgatga ttggttcccg cttttatgtg ataactcggc gtatgttatt
     3841 caagaatata taaaatcaaa tcggtataaa tctgaccgat agattttgaa tttaagagtg
     3901 tcacaagaca ctcttttttc gcaccaacga aaactggttt aagccgactg cgcaaaagac
     3961 ataatcgata agctttaatg cggtagttta tcacagttaa attgctaacg cagtcaggca
     4021 ccgtgtatga aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct
     4081 gtaggcatag gcttggttat gccggtactg ccgggcctct tgcgggatcg aggcctttag
     4141 catattatgt tgccaactgt cggaacgaga cttctctaaa ccttcccggc ttcatcatgc
     4201 tctcttaaca ctgctacatc aaccccttga atcctcaatt cacctcctcg aaaacatttc
     4261 ccatcaatca tattctggta tatcttatct tctggcagtg acagcgtcac ttcgtaatca
     4321 ttatgattga tgataatcaa atacttccat tcatcagtct ctctttgttg cacctctaca
     4381 ttttcagcta cttcaagaat gggattaata tgatgtttag cgaaaacctg ttctaaaagc
     4441 ctgcctaaat aattactatc tggataagta ccgacgtaaa tcccctctcc tttaccgtag
     4501 cagttacgtg taaccgccgg aagtcctgca taccaatccc ccttaaatgt cgctagaggt
     4561 tctgcccctt ctaatcggat tatgtccgcc cacgtcgtac aatcatattc cccatcgtta
     4621 ctatatattt tgtttacctt tgtttctggg tatggtacaa attcctcaac aaaaatcccc
     4681 aaaatatctc gcagaggacc aggatatccg cctagatgta cacggtcatt ttcatctaca
     4741 atgccactga agaaactgac aatcaaagtg ccaccgttag caacaaattg ccgtaagttt
     4801 tcatcttctc cctctttaac catatataac attggagcaa taactacttt gtattttgtt
     4861 agatcatcag atggccttac aaaatcgaca gcaatattac gtttatataa ttccctataa
     4921 taagcttcaa ctataggaat atatcttagt ttattatgtg gtttggaact tagttcgaca
     4981 gcccaccagt tttcccaatc aaaaatgatc gcgacctctg ccttgattct agatccgacc
     5041 aaacaatcta actttttcag ctcttgccct aactgtgtaa cttccctata aattctatta
     5101 ttctcgttca aaaagtgggg caccattgca ccgtggaatt tttcagctcc tgctctactt
     5161 tgacgccact ggaaaaacat aataccatct gcaccacggg caatagttgc ataactccat
     5221 agacgcatta cacctggcgg ttttggaaca ttaatatcgc gccagttaac atgtgaggtt
     5281 acctgctcca tcaaaataaa cggttgacct tttcttaaac tacgcataag gtcattcatc
     5341 atggcgtgct gaattggcaa gccctctctg ggatcaggat atgagtccca tgtcacaata
     5401 tctacatgct gagcccattg aaaatagttt aacggtttga atgaacccat gaaattagtt
     5461 gatactggaa tatctggtgt tacctcacgt aaaatttcct tttctgttaa aaacaacttg
     5521 agaattgagt cattcataaa acggtagtaa tcaagttctt gggatggatt aataaaagtt
     5581 ggtgcctttc tagggggatt aatttcatcc caatgattgt atcgctgtcc ccaaaagttt
     5641 gtaccccaac gttcatttaa ttcatcgatt gttttatatc tttcctttag ccactttcta
     5701 aacgcgacag cacaattctc acaaaaacac ttggaaacgt gacatgcata ctcattatta
     5761 acatgccaca ttttgagtgc cggatgattt ttataccgtt ctgctatagc cctcacaagt
     5821 ctctttatgt gcgtaattaa ttgaggatga ttaggacaat aatgttgtct actgccaaac
     5881 gagagaatga caccgctttc atcgatcggc aaagaatctg gatacttttt tacaaaccaa
     5941 gctggagtag ttgcagtcgc cgtccccaag ttaatataaa caccgtggtc atatagtata
     6001 tctataacct tgtctagcca ttcaaagtcg aacactccat cagacggttc gatcttgctc
     6061 caactgaaaa tccctaaaga tactaaattc acccccgctt tttgcatcaa cttagcatct
     6121 tcataccaaa tttcctctgg ccattgctct gggttataat ctcctccgta acaaattgag
     6181 gataacacat tcataattat tccccctagc taattttcgt ttaattataa attaagttaa
     6241 aatttaggta cctacgtagg atcgatccga ccttaattat attatagtcc caatataatc
     6301 atttatcaac tcttttacac ttaaatttcc taatatttgt aaatattttt ttgaataaaa
     6361 aaatctagct aatgttacgt tacacattaa ctagacagat ctatcgatgc atgccatggt
     6421 acccgggagc tcgaattcga gaccgctagc gcggccgcgg gcccctcgag ggtctcggat
     6481 ccgatatcgc ccgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg
     6541 cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca
     6601 gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca ttggaccgct
     6661 gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt
     6721 aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc
     6781 cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac tccaagaatt
     6841 ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg gcagaacata
     6901 tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcgggcag cgttgggtcc
     6961 tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
     7021 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc
     7081 tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa
     7141 agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg catcgcagga
     7201 tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg gcattgaccc
     7261 tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc ctcacaacgt
     7321 tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct ctctcgtttc
     7381 atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc agtgaccaaa
     7441 caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt aacgcttctg
     7501 gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc gcttcacgac
     7561 cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     7621 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     7681 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     7741 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     7801 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     7861 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7921 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7981 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8041 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8101 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8161 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8221 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8281 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8341 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8401 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8461 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     8521 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8581 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8641 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8701 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8761 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8821 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8881 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8941 tgataccgcg agatccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9001 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9061 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9121 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9181 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9241 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9301 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9361 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9421 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9481 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9541 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9601 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9661 gaatactcat
//
