pLacCOs1 vector (V005085)

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LOCUS       V005085                 9287 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005085
VERSION     V005085
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9287)
  AUTHORS   Oster CJ, Phillips GJ.
  TITLE     Vectors for ligation-independent construction of lacZ gene fusions
            and cloning of PCR products using a nicking endonuclease
  JOURNAL   Plasmid 66 (3), 180-185 (2011)
   PUBMED   21854804
REFERENCE   2  (bases 1 to 9287)
  AUTHORS   Oster C, Phillips GJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2011) Veterinary Microbiology, Iowa State
            University, 1802 University Boulevard, Ames, IA 50010, USA
REFERENCE   3  (bases 1 to 9287)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9287)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid";
            date: "2011"; volume: "66"; issue: "3"; pages: "180-185"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-APR-2011) Veterinary Microbiology, Iowa State University, 1802
            University Boulevard, Ames, IA 50010, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             70..3114
                     /label="lacZ"
                     /note="beta-galactosidase"
     CDS             3169..4419
                     /gene="lacY"
                     /label="Lactose permease"
                     /note="Lactose permease from Escherichia coli (strain K12).
                     Accession#: P02920"
     CDS             5982..6170
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    6275..6415
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6601..7189)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7363..8220)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8221..8325)
                     /label="AmpR promoter"
     terminator      8452..8538
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      8633..8719
                     /gene="Escherichia coli rrnB"
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      8814..8900
                     /gene="Escherichia coli rrnB"
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(9000..9043)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    9174..9230
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"