LOCUS       V005085                 9287 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005085
VERSION     V005085
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9287)
  AUTHORS   Oster CJ, Phillips GJ.
  TITLE     Vectors for ligation-independent construction of lacZ gene fusions
            and cloning of PCR products using a nicking endonuclease
  JOURNAL   Plasmid 66 (3), 180-185 (2011)
   PUBMED   21854804
REFERENCE   2  (bases 1 to 9287)
  AUTHORS   Oster C, Phillips GJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2011) Veterinary Microbiology, Iowa State
            University, 1802 University Boulevard, Ames, IA 50010, USA
REFERENCE   3  (bases 1 to 9287)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9287)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid";
            date: "2011"; volume: "66"; issue: "3"; pages: "180-185"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-APR-2011) Veterinary Microbiology, Iowa State University, 1802
            University Boulevard, Ames, IA 50010, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             70..3114
                     /label="lacZ"
                     /note="beta-galactosidase"
     CDS             3169..4419
                     /gene="lacY"
                     /label="Lactose permease"
                     /note="Lactose permease from Escherichia coli (strain K12).
                     Accession#: P02920"
     CDS             5982..6170
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    6275..6415
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6601..7189)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7363..8220)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8221..8325)
                     /label="AmpR promoter"
     terminator      8452..8538
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      8633..8719
                     /gene="Escherichia coli rrnB"
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      8814..8900
                     /gene="Escherichia coli rrnB"
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(9000..9043)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    9174..9230
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
ORIGIN
        1 atcttcgctc ttcctcgaca ttcaggcctg cgagagttga agagctgcaa gcttgggggg
       61 atcgatcccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
      121 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
      181 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca
      241 ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc
      301 gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa cgtaacctat
      361 cccattacgg tcaatccgcc gtttgttccc acggagaatc cgacgggttg ttactcgctc
      421 acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc
      481 gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagt
      541 cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg
      601 gtgatggtgc tgcgttggag tgacggcagt tatctggaag atcaggatat gtggcggatg
      661 agcggcattt tccgtgacgt ctcgttgctg cataaaccga ctacacaaat cagcgatttc
      721 catgttgcca ctcgctttaa tgatgatttc agccgcgctg tactggaggc tgaagttcag
      781 atgtgcggcg agttgcgtga ctacctacgg gtaacagttt ctttatggca gggtgaaacg
      841 caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtggttat
      901 gccgatcgcg tcacactacg tctgaacgtc gaaaacccga aactgtggag cgccgaaatc
      961 ccgaatctct atcgtgcggt ggttgaactg cacaccgccg acggcacgct gattgaagca
     1021 gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac
     1081 ggcaagccgt tgctgattcg aggcgttaac cgtcacgagc atcatcctct gcatggtcag
     1141 gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac
     1201 gccgtgcgct gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgctac
     1261 ggcctgtatg tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt
     1321 ctgaccgatg atccgcgctg gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag
     1381 cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc
     1441 gctaatcacg acgcgctgta tcgctggatc aaatctgtcg atccttcccg cccggtgcag
     1501 tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc
     1561 gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg
     1621 ctacctggag agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt
     1681 cttggcggtt tcgctaaata ctggcaggcg tttcgtcagt atccccgttt acagggcggc
     1741 ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaacgg caacccgtgg
     1801 tcggcttacg gcggtgattt tggcgatacg ccgaacgatc gccagttctg tatgaacggt
     1861 ctggtctttg ccgaccgcac gccgcatcca gcgctgacgg aagcaaaaca ccagcagcag
     1921 tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt
     1981 catagcgata acgagctcct gcactggatg gtggcgctgg atggtaagcc gctggcaagc
     2041 ggtgaagtgc ctctggatgt cgctccacaa ggtaaacagt tgattgaact gcctgaacta
     2101 ccgcagccgg agagcgccgg gcaactctgg ctcacagtac gcgtagtgca accgaacgcg
     2161 accgcatggt cagaagccgg gcacatcagc gcctggcagc agtggcgtct ggcggaaaac
     2221 ctcagtgtga cgctccccgc cgcgtcccac gccatcccgc atctgaccac cagcgaaatg
     2281 gatttttgca tcgagctggg taataagcgt tggcaattta accgccagtc aggctttctt
     2341 tcacagatgt ggattggcga taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc
     2401 cgtgcaccgc tggataacga cattggcgta agtgaagcga cccgcattga ccctaacgcc
     2461 tgggtcgaac gctggaaggc ggcgggccat taccaggccg aagcagcgtt gttgcagtgc
     2521 acggcagata cacttgctga tgcggtgctg attacgaccg ctcacgcgtg gcagcatcag
     2581 gggaaaacct tatttatcag ccggaaaacc taccggattg atggtagtgg tcaaatggcg
     2641 attaccgttg atgttgaagt ggcgagcgat acaccgcatc cggcgcggat tggcctgaac
     2701 tgccagctgg cgcaggtagc agagcgggta aactggctcg gattagggcc gcaagaaaac
     2761 tatcccgacc gccttactgc cgcctgtttt gaccgctggg atctgccatt gtcagacatg
     2821 tataccccgt acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat
     2881 tatggcccac accagtggcg cggcgacttc cagttcaaca tcagccgcta cagtcaacag
     2941 caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat
     3001 atcgacggtt tccatatggg gattggtggc gacgactcct ggagcccgtc agtatcggcg
     3061 gaattccagc tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataataa
     3121 taaccgggca ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta
     3181 aaaaacacaa acttttggat gttcggttta ttctttttct tttacttttt tatcatggga
     3241 gcctacttcc cgtttttccc gatttggcta catgacatca accatatcag caaaagtgat
     3301 acgggtatta tttttgccgc tatttctctg ttctcgctat tattccaacc gctgtttggt
     3361 ctgctttctg acaaactcgg gctgcgcaaa tacctgctgt ggattattac cggcatgtta
     3421 gtgatgtttg cgccgttctt tatttttatc ttcgggccac tgttacaata caacatttta
     3481 gtaggatcga ttgttggtgg tatttatcta ggcttttgtt ttaacgccgg tgcgccagca
     3541 gtagaggcat ttattgagaa agtcagccgt cgcagtaatt tcgaatttgg tcgcgcgcgg
     3601 atgtttggct gtgttggctg ggcgctgtgt gcctcgattg tcggcatcat gttcaccatc
     3661 aataatcagt ttgttttctg gctgggctct ggctgtgcac tcatcctcgc cgttttactc
     3721 tttttcgcca aaacggatgc gccctcttct gccacggttg ccaatgcggt aggtgccaac
     3781 cattcggcat ttagccttaa gctggcactg gaactgttca gacagccaaa actgtggttt
     3841 ttgtcactgt atgttattgg cgtttcctgc acctacgatg tttttgacca acagtttgct
     3901 aatttcttta cttcgttctt tgctaccggt gaacagggta cgcgggtatt tggctacgta
     3961 acgacaatgg gcgaattact taacgcctcg attatgttct ttgcgccact gatcattaat
     4021 cgcatcggtg ggaaaaacgc cctgctgctg gctggcacta ttatgtctgt acgtattatt
     4081 ggctcatcgt tcgccacctc agcgctggaa gtggttattc tgaaaacgct gcatatgttt
     4141 gaagtaccgt tcctgctggt gggctgcttt aaatatatta ccagccagtt tgaagtgcgt
     4201 ttttcagcga cgatttatct ggtctgtttc tgcttcttta agcaactggc gatgattttt
     4261 atgtctgtac tggcgggcaa tatgtatgaa agcatcggtt tccagggcgc ttatctggtg
     4321 ctgggtctgg tggcgctggg cttcacctta atttccgtgt tcacgcttag cggccccggc
     4381 ccgctttccc tgctgcgtcg tcaggtgaat gaagtcgctt aagcaatcaa tgtcggatgc
     4441 ggcgcgacgc ttatccgacc aacatatcat aacggagtga tcgcattgaa catgccaatg
     4501 accgaaagaa taagagcagg caagctattt accgatatgt gcgaaggctt accggaaaaa
     4561 agacttcgtg ggaaaacgtt aatgtatgag tttaatcact cgcatccatc agaagttgaa
     4621 aaaagagaaa gcctgattaa agaaatgttt gccacggtag gggaaaacgc ctgggtagaa
     4681 ccgcctgtct atttctctta cggttccaac atccatatag gccgcaattt ttatgcaaat
     4741 ttcaatttaa ccattgtcga tgactacacg gtaacaatcg gtgataacgt actgattgca
     4801 cccaacgtta ctctttccgt tacgggacac cctgtacacc atgaattgag aaaaaacggc
     4861 gagatgtact cttttccgat aacgattggc aataacgtct ggatcggaag tcatgtggtt
     4921 attaatccag gcgtcaccat cggggataat tctgttattg gcgcgggtag tatcgtcaca
     4981 aaagacattc caccaaacgt cgtggcggct ggcgttcctt gtcgggttat tcgctcgagc
     5041 gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat
     5101 gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca
     5161 aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc
     5221 gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct
     5281 ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg
     5341 aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa
     5401 ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc
     5461 gccatctcca gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg
     5521 cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt
     5581 tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct
     5641 gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg
     5701 cggaagtcag cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta
     5761 ccctgtggaa cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt
     5821 ctctggtccc gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg
     5881 gcatgttcat catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt
     5941 acccccatga acagaaattc ccccttacac ggaggcatca agtgaccaaa caggaaaaaa
     6001 ccgcccttaa catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca
     6061 acgagctgga cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg
     6121 agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     6181 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     6241 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
     6301 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     6361 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
     6421 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6481 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6541 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6601 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6661 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6721 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6781 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6841 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6901 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6961 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7021 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     7081 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     7141 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     7201 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7261 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7321 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7381 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7441 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7501 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7561 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7621 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg
     7681 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7741 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7801 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7861 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7921 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg
     7981 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8041 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     8101 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     8161 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     8221 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     8281 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8341 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     8401 tatcacgagg ccctttcgtc ttcaagaatt ttaattccca attccaggca tcaaataaaa
     8461 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     8521 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga
     8581 gggtggcggg caggacgccc gccataaact gccaggaatt aattccaggc atcaaataaa
     8641 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     8701 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
     8761 agggtggcgg gcaggacgcc cgccataaac tgccaggaat taattccagg catcaaataa
     8821 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     8881 ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg
     8941 gagggtggcg ggcaggacgc ccgccataaa ctgccaggaa ttaattcccc aggcatcaaa
     9001 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     9061 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc
     9121 ccggagggtg gcgggcagga cgcccgccat aaactgccag gaattgggga tcggaattcg
     9181 agctcggtac ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggtaccgagc
     9241 tcggatccac tagtaacggc cgccagtgtg ctggaattcg gctttag
//
