pLenti6/BLOCK-iT-DEST vector (V011784)

LOCUS       V011784                 8675 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011784
VERSION     V011784
KEYWORDS    pLenti6 BLOCK-iT-DEST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8675)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     protein_bind    1806..1826
                     /label="attB4"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     protein_bind    1868..1992
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2424..2726)
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(3071..3727)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3728..3830)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(4008..4132)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4185..4226
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     promoter        4268..4597
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        4645..4692
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             4711..5106
                     /label="BSD"
                     /note="blasticidin S deaminase"
     LTR             5196..5429
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5501..5635
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5662..5797
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(5818..5836)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5846..5862)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6004..6459
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6485..6589
                     /label="AmpR promoter"
     CDS             6590..7447
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7621..8209
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8497..8518
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8533..8563
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8571..8587
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8595..8611
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8632..8650
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"