LOCUS       V011784                 8675 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011784
VERSION     V011784
KEYWORDS    pLenti6 BLOCK-iT-DEST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8675)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     protein_bind    1806..1826
                     /label="attB4"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     protein_bind    1868..1992
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2424..2726)
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(3071..3727)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3728..3830)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(4008..4132)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4185..4226
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     promoter        4268..4597
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        4645..4692
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             4711..5106
                     /label="BSD"
                     /note="blasticidin S deaminase"
     LTR             5196..5429
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5501..5635
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5662..5797
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(5818..5836)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5846..5862)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6004..6459
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6485..6589
                     /label="AmpR promoter"
     CDS             6590..7447
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7621..8209
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8497..8518
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8533..8563
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8571..8587
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8595..8611
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8632..8650
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcgctcaact ttgtatagaa aagttgacta gtatcgatgc gttaacgttc gaattctgca
     1861 gatatcaaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa
     1921 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
     1981 ccagtcacta tgaatcaact acttagatgg tattagtgac ctgtagtcga ccgacagcct
     2041 tccaaatgtt cttcgggtga tgctgccaac ttagtcgacc gacagccttc caaatgttct
     2101 tctcaaacgg aatcgtcgta tccagcctac tcgctattgt cctcaatgcc gtattaaatc
     2161 ataaaaagaa ataagaaaaa gaggtgcgag cctctttttt gtgtgacaaa ataaaaacat
     2221 ctacctattc atatacgcta gtgtcatagt cctgaaaatc atctgcatca agaacaattt
     2281 cacaactctt atacttttct cttacaagtc gttcggcttc atctggattt tcagcctcta
     2341 tacttactaa acgtgataaa gtttctgtaa tttctactgt atcgacctgc agactggctg
     2401 tgtataaggg agcctgacat ttatattccc cagaacatca ggttaatggc gtttttgatg
     2461 tcattttcgc ggtggctgag atcagccact tcttccccga taacggagac cggcacactg
     2521 gccatatcgg tggtcatcat gcgccagctt tcatccccga tatgcaccac cgggtaaagt
     2581 tcacgggaga ctttatctga cagcagacgt gcactggcca gggggatcac catccgtcgc
     2641 ccgggcgtgt caataatatc actctgtaca tccacaaaca gacgataacg gctctctctt
     2701 ttataggtgt aaaccttaaa ctgcatttca ccagtccctg ttctcgtcag caaaagagcc
     2761 gttcatttca ataaaccggg cgacctcagc catcccttcc tgattttccg ctttccagcg
     2821 ttcggcacgc agacgacggg cttcattctg catggttgtg cttaccagac cggagatatt
     2881 gacatcatat atgccttgag caactgatag ctgtcgctgt caactgtcac tgtaatacgc
     2941 tgcttcatag cacacctctt tttgacatac ttcgggtata catatcagta tatattctta
     3001 taccgcaaaa atcagcgcgc aaatacgcat actgttatct ggcttttagt aagccggatc
     3061 cacgcgatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     3121 tgccgacatg gaagccatca cagacggcat gatgaacctg aatcgccagc ggcatcagca
     3181 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     3241 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     3301 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     3361 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     3421 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     3481 ccagctcacc gtctttcatt gccatacgga attccggatg agcattcatc aggcgggcaa
     3541 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     3601 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     3661 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     3721 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     3781 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt
     3841 ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg gacaccagga tttatttatt
     3901 ctgcgaagtg atcttccgtc acaggtattt attcggcgca aagtgcgtcg ggtgatgctg
     3961 ccaacttagt cgactacagg tcactaatac catctaagta gttgattcat agtgactgga
     4021 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
     4081 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gttgatatcc
     4141 agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta
     4201 accctctcct cggtctcgat tctacgcgta ccggttagta atgagtttgg aattaattct
     4261 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4321 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     4381 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     4441 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4501 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     4561 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     4621 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     4681 gtataatacg acaaggtgag gaactaaacc atggccaagc ctttgtctca agaagaatcc
     4741 accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc
     4801 gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat
     4861 tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct
     4921 ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc
     4981 ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac
     5041 agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg
     5101 gagggctaag cacaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt
     5161 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     5221 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     5281 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5341 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5401 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     5461 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     5521 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5581 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     5641 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5701 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     5761 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     5821 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     5881 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     5941 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     6001 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     6061 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     6181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     6241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     6301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     6361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     6421 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     6481 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     6541 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     6601 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6661 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6721 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6781 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6841 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6901 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6961 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     7021 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     7081 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     7141 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     7201 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     7261 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     7321 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     7381 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     7441 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     7501 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     7561 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7621 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7681 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7741 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     7801 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7861 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7921 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7981 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8041 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8101 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8161 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8221 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8281 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     8341 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     8401 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     8461 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     8521 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     8581 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     8641 tcactaaagg gaacaaaagc tggagctgca agctt
//
