pMIB/V5-His A vector (V011609)

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Basic Vector Information

Vector Name:: pMIB/V5-His A

Antibiotic Resistance:: Ampicillin

Length:: 3596 bp

Type:: Insect Cell Vectors

Replication origin:: ori

Source/Author:: Invitrogen (Life Technologies)

Selection Marker:: Blasticidin

Copy Number:: High copy number

Promoter:: OpIE-2

5' Primer:: OpIE2-F: 5'd[CGCAACGATCTGGTAAACAC]3'

Fusion Tag:: HBM (Nterm), V5 (Cterm), His (Cterm)

pMIB/V5-His A vector Map

Copy Sequence View Map

pMIB/V5-His A vector Sequence

LOCUS       pMIB_V5-His_A.        3596 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Insect cell vector for expression of secreted, C-terminally 
            V5-6xHis-tagged proteins. For other reading frames, use pMIB/V5-His 
            B or pMIB/V5-His C.
ACCESSION   .
VERSION     .
KEYWORDS    pMIB V5-His A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..548
                     /label=OpIE-2 promoter
                     /note="strong constitutive baculovirus promoter for insect
                     cell expression"
     sig_peptide     565..627
                     /label=melittin signal sequence
                     /note="signal sequence from honeybee melittin"
     misc_feature    629..721
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             734..775
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             785..802
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    820..949
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     rep_origin      complement(1077..1665)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1740..2031
                     /label=OpIE-1 promoter
                     /note="moderate constitutive baculovirus promoter for
                     insect cell expression"
     promoter        2057..2104
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2123..2518
                     /label=BSD
                     /note="blasticidin S deaminase"
     CDS             2641..3498
                     /label=AmpR
                     /note="beta-lactamase"

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