LOCUS       pMIB_V5-His_A.        3596 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Insect cell vector for expression of secreted, C-terminally 
            V5-6xHis-tagged proteins. For other reading frames, use pMIB/V5-His 
            B or pMIB/V5-His C.
ACCESSION   .
VERSION     .
KEYWORDS    pMIB V5-His A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..548
                     /label=OpIE-2 promoter
                     /note="strong constitutive baculovirus promoter for insect
                     cell expression"
     sig_peptide     565..627
                     /label=melittin signal sequence
                     /note="signal sequence from honeybee melittin"
     misc_feature    629..721
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             734..775
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             785..802
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    820..949
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     rep_origin      complement(1077..1665)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1740..2031
                     /label=OpIE-1 promoter
                     /note="moderate constitutive baculovirus promoter for
                     insect cell expression"
     promoter        2057..2104
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2123..2518
                     /label=BSD
                     /note="blasticidin S deaminase"
     CDS             2641..3498
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 catgatgata aacaatgtat ggtgctaatg ttgcttcaac aacaattctg ttgaactgtg
       61 ttttcatgtt tgccaacaag cacctttata ctcggtggcc tccccaccac caactttttt
      121 gcactgcaaa aaaacacgct tttgcacgcg ggcccataca tagtacaaac tctacgtttc
      181 gtagactatt ttacataaat agtctacacc gttgtatacg ctccaaatac actaccacac
      241 attgaacctt tttgcagtgc aaaaaagtac gtgtcggcag tcacgtaggc cggccttatc
      301 gggtcgcgtc ctgtcacgta cgaatcacat tatcggaccg gacgagtgtt gtcttatcgt
      361 gacaggacgc cagcttcctg tgttgctaac cgcagccgga cgcaactcct tatcggaaca
      421 ggacgcgcct ccatatcagc cgcgcgttat ctcatgcgcg tgaccggaca cgaggcgccc
      481 gtcccgctta tcgcgcctat aaatacagcc cgcaacgatc tggtaaacac agttgaacag
      541 catctgttcg aatttaaagc taccatgaaa ttcttagtca acgttgccct tgtttttatg
      601 gtcgtataca tttcttacat ctatgccggc atgctaagct tggtaccgag ctcggatcca
      661 ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc tcgagtctag
      721 agggcccttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac
      781 cggtcatcat caccatcacc attgagttta tctgactaaa tcttagtttg tattgtcatg
      841 ttttaataca atatgttatg tttaaatatg tttttaataa attttataaa ataatttcaa
      901 cttttattgt aacaacattg tccatttaca cactcctttc aagcgcgtgg gatcgatgct
      961 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     1021 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1081 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1141 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1201 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1261 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1321 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1381 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1441 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1501 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1561 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1621 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1681 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1741 cgaaacacgc acggcgcgcg cacgcagctt agcacaaacg cgtcgttgca cgcgcccacc
     1801 gctaaccgca ggccaatcgg tcggccggcc tcatatccgc tcaccagccg cgtcctatcg
     1861 ggcgcggctt ccgcgcccat tttgaataaa taaacgataa cgccgttggt ggcgtgaggc
     1921 atgtaaaagg ttacatcatt atcttgttcg ccatccggtt ggtataaata gacgttcatg
     1981 ttggtttttg tttcagttgc aagttggctg cggcgcgcgc agcacctttg ccgggatctg
     2041 ccgggctgca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     2101 cgacaaggtg aggaactaaa ccatggccaa gcctttgtct caagaagaat ccaccctcat
     2161 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag
     2221 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
     2281 gggaccttgc gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
     2341 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
     2401 ccgacaggtt cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
     2461 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta
     2521 agcacttcgt ggccgaggag caggactgac acgtcccggg agatctgcat gtctactaaa
     2581 ctcacaaatt agagcttcaa tttaattata tcagttatta cccattgaaa aaggaagagt
     2641 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     2701 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     2761 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     2821 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     2881 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     2941 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     3001 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     3061 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     3121 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     3181 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     3241 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     3301 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     3361 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     3421 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     3481 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     3541 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatct
//
