pCRE-MetLuc2-Reporter vector (V011551)

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LOCUS       pCRE-MetLuc2-Rep        4489 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for monitoring cAMP-mediated signal transduction using 
            secreted Metridia luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pCRE-MetLuc2-Reporter
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    48..113
                     /label=bind
                     /bound_moiety="cAMP response element-binding protein CREB"
                     /note="CRE"
                     /note="cyclic AMP response element (3 copies)"
     promoter        130..278
                     /label=TATA like promoter Ptal
                     /note="TATA-like promoter region"
                     /note="TATA-like promoter region from the herpes simplex
                     virus thymidine kinase promoter"
     CDS             336..992
                     /label=MetLuc
                     /note="secreted Metridia luciferase"
     polyA_signal    1117..1238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1245..1700)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1727..1831
                     /label=AmpR promoter
     promoter        1833..2190
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2225..3016
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3251..3298
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3627..4215
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    4277..4325
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4339..4430
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"