LOCUS       pCRE-MetLuc2-Rep        4489 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for monitoring cAMP-mediated signal transduction using 
            secreted Metridia luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pCRE-MetLuc2-Reporter
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    48..113
                     /label=bind
                     /bound_moiety="cAMP response element-binding protein CREB"
                     /note="CRE"
                     /note="cyclic AMP response element (3 copies)"
     promoter        130..278
                     /label=TATA like promoter Ptal
                     /note="TATA-like promoter region"
                     /note="TATA-like promoter region from the herpes simplex
                     virus thymidine kinase promoter"
     CDS             336..992
                     /label=MetLuc
                     /note="secreted Metridia luciferase"
     polyA_signal    1117..1238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1245..1700)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1727..1831
                     /label=AmpR promoter
     promoter        1833..2190
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2225..3016
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3251..3298
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3627..4215
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    4277..4325
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4339..4430
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 tagttattac tagcgctacc ggactcagat ctcgaggcac cagacagtga cgtcagctgc
       61 cagatcccat ggccgtcata ctgtgacgtc tttcagacac cccattgacg tcaatgggag
      121 aacagatctg ccgccccgac tgcatctgcg tgttcgaatt cgccaatgac aagacgctgg
      181 gcggggtttg tgtcatcata gaactaaaga catgcaaata tatttcttcc ggggacaccg
      241 ccagcaaacg cgagcaacgg gccacgggga tgaagcagaa gcttcgaatt ctgcagtcga
      301 cggtaccgcg ggcccgggat ccaccggtcg ccaccatgga catcaaggtg gtgttcaccc
      361 tggtgttcag cgccctggtg caggccaaga gcaccgagtt cgaccccaac atcgacatcg
      421 tgggcctgga aggcaagttc ggcatcacca acctggaaac cgacctgttc accatctggg
      481 agaccatgga agtgatgatc aaggccgaca tcgccgacac cgaccgggcc agcaacttcg
      541 tggccaccga gaccgacgcc aaccggggca agatgcccgg caagaagctg cccctggccg
      601 tcatcatgga aatggaagcc aacgccttca aggccggctg cacccggggc tgcctgatct
      661 gcctgagcaa gatcaagtgc accgccaaga tgaaggtgta catccccggc aggtgccacg
      721 actacggcgg cgacaagaaa accggccagg ccggcatcgt gggcgccatc gtggacatcc
      781 ccgagatcag cggcttcaaa gaaatggccc ccatggaaca gttcatcgcc caggtggaca
      841 gatgcgccag ctgcaccacc ggctgcctga agggcctggc caacgtgaag tgcagcgagc
      901 tgctgaagaa gtggctgccc gaccgctgcg ccagcttcgc cgacaagatc cagaaagagg
      961 tgcacaacat caagggcatg gccggcgaca ggtgagcggc cgcgactcta gatcataatc
     1021 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     1081 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     1141 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     1201 tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa
     1261 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     1321 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     1381 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     1441 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     1501 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     1561 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     1621 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     1681 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     1741 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1801 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
     1861 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     1921 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     1981 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     2041 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     2101 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     2161 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
     2221 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     2281 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     2341 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     2401 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     2461 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     2521 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     2581 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     2641 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     2701 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     2761 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     2821 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     2881 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     2941 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     3001 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     3061 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     3121 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     3181 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     3241 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
     3301 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     3361 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     3421 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
     3481 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     3541 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     3601 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3661 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3721 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3781 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3841 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3901 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3961 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4021 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4081 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4141 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4201 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4261 ccttttgctc acatgtaata aaatatcttt attttcatta catctgtgtg ttggtttttt
     4321 gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact
     4381 agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct acatgttctt
     4441 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
