Basic Vector Information
- Vector Name:
- pGL4.51[luc2/CMV/Neo]
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6358 bp
- Type:
- Luciferase Vectors
- Replication origin:
- ori
- Source/Author:
- Promega
- Selection Marker:
- Neomycin/G418(Geneticin)
- Copy Number:
- High copy number
- Promoter:
- CMV
pGL4.51[luc2/CMV/Neo] vector Map
pGL4.51[luc2/CMV/Neo] vector Sequence
LOCUS pGL4.51[luc2_CMV 6358 bp DNA circular SYN 01-JAN-1980
DEFINITION Vector for creating stable cell lines expressing firefly luciferase
under control of the CMV promoter.
ACCESSION .
VERSION .
KEYWORDS pGL4.51[luc2 CMV Neo]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6358)
AUTHORS Promega
TITLE Direct Submission
REFERENCE 2 (bases 1 to 6358)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6358
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 152..530
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 531..734
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 859..2508
/label=luciferase
/note="firefly luciferase"
polyA_signal complement(2555..2676)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 2870..3227
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 3258..4049
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
polyA_signal 4077..4125
/label=poly(A) signal
/note="synthetic polyadenylation signal"
rep_origin complement(4452..5040)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5243..6100)
/label=AmpR
/note="beta-lactamase"
polyA_signal 6205..6253
/label=synthetic polyadenylation signal
/note="synthetic polyadenylation signal"
misc_feature 6267..6358
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
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