LOCUS       pGL4.51[luc2_CMV        6358 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for creating stable cell lines expressing firefly luciferase 
            under control of the CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.51[luc2 CMV Neo]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6358)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        152..530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        531..734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             859..2508
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(2555..2676)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2870..3227
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3258..4049
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4077..4125
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4452..5040)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5243..6100)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    6205..6253
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    6267..6358
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gcctcaatat tggccattag ccatattatt cattggttat atagcataaa
       61 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
      121 ttggctcatg tccaatatga ccgccatgtt ggcattgatt attgactagt tattaatagt
      181 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      241 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      301 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      361 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
      421 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
      481 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      541 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      601 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      661 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      721 atataagcag agctcgttta gtgaaccgtc agatcactag aagctttatt gcggtagttt
      781 atcacagtta aattgctaac gcagtcagtg ggcctcggcg gccaagcttg gcaatccggt
      841 actgttggta aagccaccat ggaagatgcc aaaaacatta agaagggccc agcgccattc
      901 tacccactcg aagacgggac cgccggcgag cagctgcaca aagccatgaa gcgctacgcc
      961 ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg aggtggacat tacctacgcc
     1021 gagtacttcg agatgagcgt tcggctggca gaagctatga agcgctatgg gctgaataca
     1081 aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt
     1141 gccctgttca tcggtgtggc tgtggcccca gctaacgaca tctacaacga gcgcgagctg
     1201 ctgaacagca tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa
     1261 aagatcctca acgtgcaaaa gaagctaccg atcatacaaa agatcatcat catggatagc
     1321 aagaccgact accagggctt ccaaagcatg tacaccttcg tgacttccca tttgccaccc
     1381 ggcttcaacg agtacgactt cgtgcccgag agcttcgacc gggacaaaac catcgccctg
     1441 atcatgaaca gtagtggcag taccggattg cccaagggcg tagccctacc gcaccgcacc
     1501 gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg gcaaccagat catccccgac
     1561 accgctatcc tcagcgtggt gccatttcac cacggcttcg gcatgttcac cacgctgggc
     1621 tacttgatct gcggctttcg ggtcgtgctc atgtaccgct tcgaggagga gctattcttg
     1681 cgcagcttgc aagactataa gattcaatct gccctgctgg tgcccacact atttagcttc
     1741 ttcgctaaga gcactctcat cgacaagtac gacctaagca acttgcacga gatcgccagc
     1801 ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg ccaaacgctt ccacctacca
     1861 ggcatccgcc agggctacgg cctgacagaa acaaccagcg ccattctgat cacccccgaa
     1921 ggggacgaca agcctggcgc agtaggcaag gtggtgccct tcttcgaggc taaggtggtg
     1981 gacttggaca ccggtaagac actgggtgtg aaccagcgcg gcgagctgtg cgtccgtggc
     2041 cccatgatca tgagcggcta cgttaacaac cccgaggcta caaacgctct catcgacaag
     2101 gacggctggc tgcacagcgg cgacatcgcc tactgggacg aggacgagca cttcttcatc
     2161 gtggaccggc tgaagagcct gatcaaatac aagggctacc aggtagcccc agccgaactg
     2221 gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg gggtcgccgg cctgcccgac
     2281 gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg aacacggtaa aaccatgacc
     2341 gagaaggaga tcgtggacta tgtggccagc caggttacaa ccgccaagaa gctgcgcggt
     2401 ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg gcaagttgga cgcccgcaag
     2461 atccgcgaga ttctcattaa ggccaagaag ggcggcaaga tcgccgtgta ataattctag
     2521 agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga gtttggacaa
     2581 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     2641 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     2701 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     2761 tgtggtaaaa tcgataagga tccgtttgcg tattgggcgc tcttccgctg atctgcgcag
     2821 caccatggcc tgaaataacc tctgaaagag gaacttggtt agctaccttc tgaggcggaa
     2881 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     2941 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     3001 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     3061 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     3121 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     3181 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcgattctt
     3241 ctgacactag cgccaccatg atcgaacaag acggcctcca tgctggcagt cccgcagctt
     3301 gggtcgaacg cttgttcggg tacgactggg cccagcagac catcggatgt agcgatgcgg
     3361 ccgtgttccg tctaagcgct caaggccggc ccgtgctgtt cgtgaagacc gacctgagcg
     3421 gcgccctgaa cgagcttcaa gacgaggctg cccgcctgag ctggctggcc accaccggcg
     3481 taccctgcgc cgctgtgttg gatgttgtga ccgaagccgg ccgggactgg ctgctgctgg
     3541 gcgaggtccc tggccaggat ctgctgagca gccaccttgc ccccgctgag aaggtttcta
     3601 tcatggccga tgcaatgcgg cgcctgcaca ccctggaccc cgctacctgc cccttcgacc
     3661 accaggctaa gcatcggatc gagcgtgctc ggacccgcat ggaggccggc ctggtggacc
     3721 aggacgacct ggacgaggag catcagggcc tggcccccgc tgaactgttc gcccgactga
     3781 aagcccgcat gccggacggt gaggacctgg ttgtcacaca cggagatgcc tgcctcccta
     3841 acatcatggt cgagaatggc cgcttctccg gcttcatcga ctgcggtcgc ctaggagttg
     3901 ccgaccgcta ccaggacatc gccctggcca cccgcgacat cgctgaggag cttggcggcg
     3961 agtgggccga ccgcttctta gtcttgtacg gcatcgcagc tcccgacagc cagcgcatcg
     4021 ccttctaccg cttgctcgac gagttctttt aatgatctag aaccggtcat ggccgcaata
     4081 aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgttcga actagatgct
     4141 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
     4201 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     4261 cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     4321 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     4381 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     4441 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     4501 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     4561 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     4621 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     4681 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4741 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4801 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4861 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     4921 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     4981 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     5041 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     5101 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     5161 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag cggccgcaaa
     5221 tgctaaacca ctgcagtggt taccagtgct tgatcagtga ggcaccgatc tcagcgatct
     5281 gcctatttcg ttcgtccata gtggcctgac tccccgtcgt gtagatcact acgattcgtg
     5341 agggcttacc atcaggcccc agcgcagcaa tgatgccgcg agagccgcgt tcaccggccc
     5401 ccgatttgtc agcaatgaac cagccagcag ggagggccga gcgaagaagt ggtcctgcta
     5461 ctttgtccgc ctccatccag tctatgagct gctgtcgtga tgctagagta agaagttcgc
     5521 cagtgagtag tttccgaaga gttgtggcca ttgctactgg catcgtggta tcacgctcgt
     5581 cgttcggtat ggcttcgttc aactctggtt cccagcggtc aagccgggtc acatgatcac
     5641 ccatattatg aagaaatgca gtcagctcct tagggcctcc gatcgttgtc agaagtaagt
     5701 tggccgcggt gttgtcgctc atggtaatgg cagcactaca caattctctt accgtcatgc
     5761 catccgtaag atgcttttcc gtgaccggcg agtactcaac caagtcgttt tgtgagtagt
     5821 gtatacggcg accaagctgc tcttgcccgg cgtctatacg ggacaacacc gcgccacata
     5881 gcagtacttt gaaagtgctc atcatcggga atcgttcttc ggggcggaaa gactcaagga
     5941 tcttgccgct attgagatcc agttcgatat agcccactct tgcacccagt tgatcttcag
     6001 catcttttac tttcaccagc gtttcggggt gtgcaaaaac aggcaagcaa aatgccgcaa
     6061 agaagggaat gagtgcgaca cgaaaatgtt ggatgctcat actcgtcctt tttcaatatt
     6121 attgaagcat ttatcagggt tactagtacg tctctcaagg ataagtaagt aatattaagg
     6181 tacgggaggt attggacagg ccgcaataaa atatctttat tttcattaca tctgtgtgtt
     6241 ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa
     6301 aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctct
//
