G Force or Relative Centrifugal Force (RCF) is the amount of acceleration to be applied to the sample. It depends on the rotations per minute (RPM) and radius of the rotor, and is relative to the force of Earth’s gravity.
A good, precise protocol for centrifugation instructs you to use the G force rather than RPMs because the rotor size might differ, and g force will be different while the revolutions per minute stay the same. Unfortunately, many protocols are written in hurry and instructions are given in RPMs. Therefore, you have to convert G force (RCF) into revolutions per minute (rpms) and vice versa.
0. Select the Brand of the centrifuge
G force RPM Conversion Formula
The G force RPM conversion formula is as follows: RCF = 1.118 * r * (rpm/1000)2
RCF = Relative Centrifuge Force, in "g"
r = Rotor radius, in "mm"
rpm = Rotation speed, rotations per minute, in "rpm"