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Product Name
SETD3 Polyclonal Antibody
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Description
Polyclonal antibody to SETD3
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Tested applications
WB, IP
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Species reactivity
Human, Mouse
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Alternative names
SETD3 antibody; C14orf154 antibody; SET domain containing 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SETD3 (NP_115609.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IP 1:50 - 1:100 -
Validations
Western blot - SETD3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SETD3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunoprecipitation - SETD3 Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug SETD3 antibody . Western blot was performed from the immunoprecipitate using SETD3 antibody at a dilition of 1:1000.
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Background
Protein-histidine N-methyltransferase that specifically mediates methylation of actin at 'His-73'. Histidine methylation of actin is required for smooth muscle contraction of the laboring uterus during delivery (Ref.13). Does not have protein-lysine N-methyltransferase activity and probably only catalyzes histidine methylation of actin (Ref.13).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"