Cynomolgus CD73 / NT5E (His Tag) recombinant protein

Cynomolgus CD73 / NT5E (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the 5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.

Putative uncharacterized protein

RP11-321N4.1; ENT; 5'-NT; CD73; EN; NT5E; 5'-NUCLEOTIDASE; AI447961; CALJA; NT5; NT; E5NT; ECTO-5'-NUCLEOTIDASE; NTE; 2210401F01RIK

G7P3J1

EGM_13811

Human Cells

A DNA sequence encoding the cynomolgus NT5E (EHH53214.1) (Met1-Lys547) was expressed with a polyhistidine tag at the C-terminus.

Cynomolgus

The recombinant cynomolgus NT5E comprises 532 amino acids and has a calculated molecular mass of 59.2 KDa. The apparent molecular mass of it is approximately 59 KDa respectively in SDS-PAGE.

> 95 % as determined by SDS-PAGE

Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996).
The specific activity is >20,000 pmol/min/µg.