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  • MED14 Polyclonal Antibody

MED14 Polyclonal Antibody

Cat.#: 160500

Special Price 174.2 USD

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Product Information

  • Product Name
    MED14 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to MED14
  • Tested applications
    WB
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    MED14 antibody; CRSP150 antibody; CRSP2 antibody; CSRP antibody; CXorf4 antibody; DRIP150 antibody; EXLM1 antibody; RGR1 antibody; TRAP170 antibody; mediator complex subunit 14 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 470-650 of human MED14 (NP_004220.2).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - MED14 Polyclonal Antibody

    Western blot - MED14 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using MED14 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.

  • Background
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"