MED14 Polyclonal Antibody
Polyclonal antibody to MED14
Human, Mouse, Rat
MED14 antibody; CRSP150 antibody; CRSP2 antibody; CSRP antibody; CXorf4 antibody; DRIP150 antibody; EXLM1 antibody; RGR1 antibody; TRAP170 antibody; mediator complex subunit 14 antibody
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 470-650 of human MED14 (NP_004220.2).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
Western blot - MED14 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MED14 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"