Cynomolgus IFNA4 (Fc Tag) recombinant protein

Cynomolgus IFNA4 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interferon, alpha 4 (IFNA4) belongs to the alpha/beta interferon family. Two variants of IFNA4 (IFNA4a and IFNA4b) are known, which differ from each other by changes in their coding regions at nucleotide positions 220 and 410 and can be distinguished by selective restriction enzyme analysis. Interferons are produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha, the first cytokine to be produced by recombinant DNA technology, has emerged as an important regulator of growth and differentiation, affecting cellular communication and signal transduction pathways as well as immunological control. Originally discovered as an antiviral substance, the efficacy of IFN-alpha in malignant, viral, immunological, angiogenic, inflammatory, and fibrotic diseases suggests a spectrum of interrelated pathophysiologies. IFN-alpha emerged as a prototypic tumor suppressor protein that represses the clinical tumorigenic phenotype in some malignancies capable of differentiation.

IFNA4; MGC142200; RP23-139P14.30; MGC143607; MUIFN-ALPHA 4; IFA4

Human Cells

A DNA sequence encoding the cynomolgus IFNA4 (Met1-Asn189) was expressed with Fc region of mouse IgG at the C-terminus.

Cynomolgus

The recombinant cynomolgus IFNA4 is a disulfide-linked homodimer. The reduced monomer comprises 400 amino acids and has a calculated molecular mass of 45.6 KDa.The apparent molecular mass of the protein is approximately 48 KDa respectively in SDS-PAGE.

> 90 % as determined by SDS-PAGE

Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitisvirus (VSV).
The ED₅₀ for this effect is 0.03-0.3 ng/mL.