Human Acetoacetyl-CoA Synthetase (His Tag) recombinant protein

Human Acetoacetyl-CoA Synthetase (His Tag) recombinant protein

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Acetoacetyl-CoA Synthetase (AACS) is a novel cytosolic ketone body (acetoacetate)-specific ligase. The AACS in adipose tissue plays an important role in utilizing ketone body for the fatty acid-synthesis during adipose tissue development. It had been improved that Acetoacetyl-CoA Synthetase is an essential enzyme for the synthesis of fatty acid and cholesterol from ketone bodies, was found to be highly expressed in mouse adipose tissue, and GC box and C/EBPs motif were crucial for AACS promoter activity in 3T3-L1 adipocytes. Moreover, AACS promoter activity was controlled mainly by C/EBPalpha during adipogenesis. AACS gene expression is particularly abundant in white adipose tissue, as it is induced during adipocyte differentiation. The human AACS promoter is a PPARgamma target gene and that this nuclear receptor is recruited to the AACS promoter by direct interaction with Sp1 (stimulating protein-1). The Acetoacetyl-CoA Synthetase has important roles in the regulation of ketone body utilization in rat liver and that these hypocholesterolemic agents have the ability to remedy the impaired utilization of ketone bodies under the diabetic condition.

Acetoacetyl-CoA synthetase, isoform CRA_b

SUR5; FLJ12389; FLJ41251; ACETOACETYL-COA SYNTHETASE; 2210408B16RIK; SUR-5; ACSF1; AACS

Q86V21

AACS; hCG_32866

Baculovirus-Insect Cells

A DNA sequence encoding the full length of human AACS isoform a (NP_076417.2) (Met 1-Phe 672) was expressed, with a polyhistidine tag at the N-terminus.

Human

The recombinant human AACS consists of 690 amino acids and predicts a molecular mass of 77 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.

> 96 % as determined by SDS-PAGE