pSHTB10 vector (V003223)

LOCUS       V003223                 7871 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003223
VERSION     V003223
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7871)
  AUTHORS   Wang Y, Su H, Li G, Yang M, Yi J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2017) School of Environmental and Biological
            Engineering, Nanjing University of Science and Technology,
            XiaoLinwei 200#, Nan Jing, JiangSu 210094, China
REFERENCE   2  (bases 1 to 7871)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7871)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (21-MAR-2017) School of Environmental and Biological Engineering,
            Nanjing University of Science and Technology, XiaoLinwei 200#, Nan
            Jing, JiangSu 210094, China"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..504
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        769..989
                     /label="URA3 promoter"
     CDS             990..1790
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(1924..2379)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2524..2540
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2550..2568
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(2587..2834)
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     CDS             complement(3379..4083)
                     /gene="SCEI"
                     /label="Intron-encoded endonuclease I-SceI"
                     /note="Intron-encoded endonuclease I-SceI from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P03882"
     protein_bind    complement(4093..4109)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(4147..4163)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(4178..4619)
                     /label="GAL1 promoter"
                     /note="inducible promoter, regulated by Gal4"
     rep_origin      4651..5181
                     /label="oriV"
                     /note="incP origin of replication"
     regulatory      5403..5521
                     /label="neo promoter"
                     /note="neo promoter"
                     /regulatory_class="promoter"
     CDS             5573..6718
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     regulatory      6772..7377
                     /regulatory_class="terminator"
     regulatory      7381..7434
                     /label="hbs promoter"
                     /note="hbs promoter"
                     /regulatory_class="promoter"
     CDS             7435..7806
                     /label="BleoR"
                     /note="antibiotic-binding protein"