pSHAFT2 vector (V003225)

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LOCUS       V003225                 4570 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003225
VERSION     V003225
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Shastri S, Spiewak HL, Sofoluwe A, Eidsvaag VA, Asghar AH, Pereira
            T, Bull EH, Butt AT, Thomas MS.
  TITLE     An efficient system for the generation of marked genetic mutants in
            members of the genus Burkholderia
  JOURNAL   Plasmid (2016) In press
   PUBMED   27825973
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Shastri S, Spiewak HL, Pereira T, Sofoluwe AO, Eidsvaag VA, Bull EH,
            Asghar AH, Thomas MS.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4570)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4570)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid
            (2016) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-JUN-2016) Department of Infection, Immunity "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             144..782
                     /gene="cmlA"
                     /label="Chloramphenicol acetyltransferase 2"
                     /note="Chloramphenicol acetyltransferase 2 from Escherichia
                     coli. Accession#: P22615"
     misc_feature    903..921
                     /label="O end"
                     /note="O end"
     protein_bind    complement(933..949)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     rep_origin      complement(984..1372)
                     /direction=LEFT
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(1993..2361)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(2394..2503)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             complement(3584..4441)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4442..4546)
                     /label="AmpR promoter"