pSG-CaGFP-1 vector (V003278)

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LOCUS       V003278                 6444 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003278
VERSION     V003278
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6444)
  AUTHORS   Galuska S, Donald RGK., Muise E, Wiersma HI, Diaz C, Kulkarni A,
            Cavet G, Roemer T, Liberator P, Douglas C.
  TITLE     A Candida albicans GFP reporter assay for identifying inhibitors of
            cell wall biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6444)
  AUTHORS   Galuska S.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6444)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6444)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-MAR-2009) Infectious Diseases, Merck "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             241..1653
                     /codon_start=1
                     /product="ARG4"
                     /label="ARG4"
                     /note="contains unique NotI site"
                     /protein_id="ADD22644.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELHLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     AADAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEP
                     LFDALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHI
                     SGECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDAIGGTAKSAV
                     LKQLENLKSILS"
     misc_feature    1081..1088
                     /note="NotI site; inserted in ARG4 to facilitate targeted
                     integration at the ARG4 locus of Candida albicans
                     transformants"
     primer_bind     1910..1926
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1972..2676
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     primer_bind     complement(2864..2880)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2888..2904)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2912..2942)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2957..2978)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3266..3854)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4028..4885)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4886..4990)
                     /label="AmpR promoter"
     CDS             5435..6244
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"