pSF_lenti_ZEBOV14_NP-VP35-GP vector (V003280)

LOCUS       V003280                11028 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003280
VERSION     V003280
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11028)
  AUTHORS   Mattiuzzo G, Ashall J, Doris KS, MacLellan-Gibson K, Nicolson C,
            Wilkinson DE, Harvey R, Almond N, Anderson R, Efstathiou S, Minor
            PD, Page M.
  TITLE     Development of Lentivirus-Based Reference Materials for Ebola Virus
            Nucleic Acid Amplification Technology-Based Assays
  JOURNAL   PLoS ONE 10 (11), E0142751 (2015)
   PUBMED   26562415
REFERENCE   2  (bases 1 to 11028)
  AUTHORS   Mattiuzzo G, Mitchell J, Mee ET.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUN-2015) Virology, National Institute for Biological
            Standards and Controls, Blanche Lane, South Mimms-Potters Bar EN6
            3QG, United Kingdom
REFERENCE   3  (bases 1 to 11028)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11028)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Geneious v. 7.1.8
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2015"; volume: "10"; issue: "11"; pages: "E0142751"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-JUN-2015) Virology, National Institute for Biological Standards
            and Controls, Blanche Lane, South Mimms-Potters Bar EN6 3QG, United
            Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     601..617
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        624..642
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        713..1016
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1017..1220
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             1235..1415
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1462..1587
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    2080..2313
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2498..2542
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2691..2732
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2809..2925
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     misc_feature    3001..3092
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     gene            complement(3097..5280)
                     /gene="NP"
                     /label="NP"
     gene            complement(5284..6153)
                     /gene="VP35"
                     /label="VP35"
     CDS             complement(7208..8284)
                     /gene="GP"
                     /label="Pre-small/secreted glycoprotein"
                     /note="Pre-small/secreted glycoprotein from Zaire
                     ebolavirus (strain Kikwit-95). Accession#: P60171"
     LTR             8386..8619
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    complement(8636..8757)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(8840..8858)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8879..8895)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    8903..8919
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8927..8957)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8972..8993)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9281..9869)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10043..10900)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10901..11005)
                     /label="AmpR promoter"