pSEAPEhBCTNf1 vector (V003389)

LOCUS       40924_39103        6210 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSEAPEhBCTNf1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6210)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6210)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      60..162
                     /label=human BCTN promoter region
                     /note="human BCTN promoter region"
                     /regulatory_class="promoter"
     misc_feature    163..201
                     /label=5' UTR of human BCTN
                     /note="5' UTR of human BCTN"
     CDS             209..1714
                     /label=SEAP
                     /note="secreted alkaline phosphatase from human placenta"
     CDS             2248..2322
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88330.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     intron          2324..2389
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2519..2539
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2964..3098
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(3103..3338)
                     /label=SV40 early enhancer
                     /note="SV40 early enhancer"
                     /regulatory_class="promoter"
     rep_origin      complement(3668..4256)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4430..5287)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5288..5392)
                     /label=AmpR promoter
     rep_origin      5419..5874
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     regulatory      complement(6042..6138)
                     /label=SV40 polyA late
                     /note="SV40 polyA late"
                     /regulatory_class="terminator"
     regulatory      6042..6138
                     /label=SV40 polyA early
                     /note="SV40 polyA early"
                     /regulatory_class="terminator"
     polyA_signal    6064..6185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"