pSEAPBhBCTNf3 vector (V003390)

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LOCUS       40924_39098       11420 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSEAPBhBCTNf3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11420)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11420)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 11420)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11420)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    52..3504
                     /label=5' flanking region of human BCTN
                     /note="5' flanking region of human BCTN"
     regulatory      3505..4499
                     /label=human BCTN promoter region
                     /note="human BCTN promoter region"
                     /regulatory_class="promoter"
     misc_feature    4500..4665
                     /label=5' UTR of human BCTN
                     /note="5' UTR of human BCTN"
     misc_feature    4666..5668
                     /label=part of intron 1 of human BCTN
                     /note="part of intron 1 of human BCTN"
     CDS             5667..7181
                     /label=SEAP
                     /note="secreted alkaline phosphatase from human placenta"
     CDS             7715..7789
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88318.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     intron          7791..7856
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7986..8006
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    8431..8565
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(8878..9466)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9640..10497)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10498..10602)
                     /label=AmpR promoter
     rep_origin      10629..11084
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    11274..11395
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"