pSE380rPDI vector (V003394)

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LOCUS       V003394                 5885 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003394
VERSION     V003394
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5885)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5885)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5885)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(43..1122)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(1123..1200)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     misc_feature    complement(1201..1231)
                     /label="5FR"
                     /note="5FR"
     misc_feature    1386..1526
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1712..2300)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2474..3331)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3332..3423)
                     /label="AmpR promoter"
     terminator      complement(3443..3470)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(3562..3648)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    complement(3649..3768)
                     /label="5SRNA"
                     /note="5SRNA"
     CDS             complement(4096..5553)
                     /gene="P4hb"
                     /label="Protein disulfide-isomerase"
                     /note="Protein disulfide-isomerase from Rattus norvegicus.
                     Accession#: P04785"
     misc_feature    complement(5563..5619)
                     /label="srPDI"
                     /note="srPDI"
     protein_bind    complement(5640..5656)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5664..5693)
                     /label="trc promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"