pSe1Bb1s-gfp vector (V003395)

Download GeneBank File(.gb)

LOCUS       V003395                 5907 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003395
VERSION     V003395
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Woo HM.
  TITLE     Application of synthetic biology platform of CyanoBrick vectors:
            analysis of the elevated CO2 concentration on biomass and gene
            expression of Synechococcus elongatus PCC7942
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   Woo HM.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2014) Clean Energy Research Center, Korea
            Institute of Science and Technology, Hwarangno 14-gil 5,
            Seongbuk-gu, Seoul 136-791, Korea
REFERENCE   3  (bases 1 to 5907)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5907)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-MAY-2014) Clean Energy Research Center, Korea Institute of
            Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul
            136-791, Korea"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      84..672
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     gene            745..1543
                     /gene="NS1a"
                     /label="NS1a"
     CDS             1903..2691
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     promoter        2721..2798
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             2799..3878
                     /label="lacI"
                     /note="lac repressor"
     promoter        4113..4142
                     /label="trc promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    4150..4166
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4207..4920
                     /label="GFPuv"
                     /note="GFP variant optimized for excitation by UV light"
     terminator      4956..5027
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      5043..5070
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     CDS             5423..5869
                     /gene="ndk"
                     /label="Nucleoside diphosphate kinase"
                     /note="Nucleoside diphosphate kinase from Synechococcus
                     elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805).
                     Accession#: P50590"