pSCUDT2MFE6H vector (V003402)

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LOCUS       V003402                13829 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003402
VERSION     V003402
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13829)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13829)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 13829)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13829)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      341..1683
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(1837..2379)
                     /gene="RAF1"
                     /label="Trans-acting factor D"
                     /note="Trans-acting factor D from Saccharomyces cerevisiae
                     (strain ATCC 204508 / S288c). Accession#: Q06891"
     CDS             2642..3760
                     /gene="REP1"
                     /label="Partitioning protein REP1"
                     /note="Partitioning protein REP1 from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P03871"
     protein_bind    complement(3927..3974)
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(4308..5195)
                     /gene="REP2"
                     /label="Partitioning protein REP2"
                     /note="Partitioning protein REP2 from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P03872"
     regulatory      6345..7326
                     /label="CYC1"
                     /note="CYC1"
                     /regulatory_class="promoter"
     CDS             7350..7910
                     /label="DHFR"
                     /note="mouse dihydrofolate reductase"
     terminator      8022..8269
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     promoter        8276..8496
                     /label="URA3 promoter"
     CDS             8497..9297
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(9462..10050)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10224..11081)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(11082..11186)
                     /label="AmpR promoter"
     misc_feature    complement(11320..11410)
                     /label="3UTR"
                     /note="3UTR"
     CDS             complement(11414..12421)
                     /label="mlgG2b-CH"
                     /note="Mouse IgG2b heavy chain constant region"
     CDS             complement(12782..13036)
                     /label="alpha-factor secretion signal"
                     /note="N-terminal secretion signal from S. cerevisiae
                     alpha-factor"
     promoter        complement(13098..13539)
                     /label="GAL1 promoter"
                     /note="inducible promoter, regulated by Gal4"
     terminator      13572..13819
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"