pSCPT vector (V003408)

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LOCUS       V003408                 8596 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003408
VERSION     V003408
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Kanda K, Ishida T, Hirota R, Ono S, Motomura K, Ikeda T, Kitamura K,
            Kuroda A.
  TITLE     Application of a phosphite dehydrogenase gene as a novel dominant
            selection marker for yeasts
  JOURNAL   J. Biotechnol. 182-183, 68-73 (2014)
   PUBMED   24786825
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   Hirota R.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2013) Contact:Ryuichi Hirota Hiroshima University,
            Department of Biotechnology; 1-3-1, Higashi-Hiroshima, Hiroshima
            739-8530, Japan
REFERENCE   3  (bases 1 to 8596)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8596)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Biotechnol. 182-183, 68-73 (2014)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-NOV-2013) Contact:Ryuichi Hirota Hiroshima University,
            Department of Biotechnology; 1-3-1, Higashi-Hiroshima, Hiroshima
            739-8530, Japan"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3695..3764
                     /mol_type="other DNA"
                     /db_xref="taxon:4932"
                     /organism="Saccharomyces cerevisiae"
     misc_feature    1..6
                     /label="EcoRI recognition site"
                     /note="EcoRI recognition site"
     rep_origin      344..1686
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
     misc_feature    2241..2246
                     /label="EcoRI recognition site"
                     /note="EcoRI recognition site"
     promoter        2278..2498
                     /label="URA3 promoter"
     CDS             2499..3299
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     misc_feature    3378..3383
                     /label="SmaI recognition site"
                     /note="SmaI recognition site"
     regulatory      3695..3764
                     /label="IPCS promoter"
                     /note="IPCS promoter"
                     /regulatory_class="promoter"
     CDS             3765..4775
                     /codon_start=1
                     /gene="OPTptxD"
                     /product="phosphite dehydrogenase"
                     /label="OPTptxD"
                     /protein_id="BAP74305.1"
                     /translation="MKPKVVLTHWVHPEIIELLSASADVIPNTTRETLPRSEVIARAKD
                     ADALMAFMPDSIDSAFLEECPKLRVIGAALKGYDNFDVNACTRHGVWLTIVPDLLTIPT
                     AELTIGLLLGLTRHMLEGDRQIRSGHFQGWRPTLYGSGLTGKTLGIIGMGAVGRAIAQR
                     LAGFEMNLLYCDPIPLNAEQEKAWHVQRVTLDELLEKCDYVVPMVPMAAETLHLIDATA
                     LAKMKTGSYLINACRGSVVDENAVIAALASGKLAGYAADVFEMEEWIRADRPQAIPKAL
                     LDNTAQTFFTPHLGSAVKEVRLEIERQAAMNIIQALAGEKPMGAINQPYPGVKAA"
     gene            3765..4775
                     /gene="OPTptxD"
                     /label="OPTptxD"
     CDS             complement(5089..5481)
                     /gene="MRP17"
                     /label="Small ribosomal subunit protein bS6m"
                     /note="Small ribosomal subunit protein bS6m from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P28778"
     CDS             6153..6341
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    6446..6586
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6772..7360)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7534..8391)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8392..8496)
                     /label="AmpR promoter"