Basic Vector Information
- Vector Name:
- pSCCA5-E8d
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4575 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Arimitsu H, Sasaki K, Iba Y, Kurosawa Y, Shimizu T, Tsuji T.
pSCCA5-E8d vector Vector Map
pSCCA5-E8d vector Sequence
LOCUS 40924_38963 4575 bp DNA circular SYN 18-DEC-2018 DEFINITION Expression vector pSCCA5-E8d DNA, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4575) AUTHORS Arimitsu H, Sasaki K, Iba Y, Kurosawa Y, Shimizu T, Tsuji T. TITLE Isolation of B subunit-specific monoclonal antibody clones that strongly neutralize the toxicity of Shiga toxin 2 JOURNAL Microbiol. Immunol. 59 (2), 71-81 (2015) PUBMED 25521016 REFERENCE 2 (bases 1 to 4575) AUTHORS Arimitsu H, Iba Y. TITLE Direct Submission JOURNAL Submitted (06-JUN-2014) Contact:Hideyuki Arimitsu Fujita Health University, Department of Microbiology; 1-98, Dengakugakubo, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL :https://fujita-hu.ac.jp/ REFERENCE 3 (bases 1 to 4575) TITLE Direct Submission REFERENCE 4 (bases 1 to 4575) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol. Immunol."; date: "2015"; volume: "59"; issue: "2"; pages: "71-81" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (06-JUN-2014) Contact:Hideyuki Arimitsu Fujita Health University, Department of Microbiology"; volume: " 1-98, Dengakugakubo, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL :https"; pages: "//fujita-hu.ac.jp" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4575 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 40..87 /label=PelB leader sequence /note="PelB leader sequence" misc_feature 197..241 /label=Single chain linker /note="Single chain linker" CDS 375..692 /codon_start=1 /label=mIg-kappa-CL /note="Mouse immunoglobulin kappa light chain constant region" /translation="ADAAPTVSIFPPSSEQLTSGGASVVCFLNSFYPKDINVKWKIDGS ERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRN EC" misc_feature 711..1340 /label=cp3 /note="cp3" CDS 1393..1566 /codon_start=1 /label=ProtA /note="IgG-binding unit of Staphylococcus aureus protein A" /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL AEAKKLNDAQAPK" CDS 1570..1740 /codon_start=1 /product="IgG-binding unit of Staphylococcus aureus protein A" /label=ProtA /translation="DNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA EAKKLNDAQAPK" primer_bind complement(1770..1786) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1928..2383) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2410..2514 /label=AmpR promoter CDS 2515..3372 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 3546..4134 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 4422..4443 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 4458..4488 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4496..4512 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 4520..4536 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 4555..4573 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase"
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