pScaf-8064.2 vector (V003431)

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LOCUS       V003431                10819 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003431
VERSION     V003431
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10819)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM.
  TITLE     Construction of a novel phagemid to produce custom DNA origami
            scaffolds
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10819)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2018) Cellular and Molecular Pharmacology,
            University of California, San Francisco, 600 16th Street, Genentech
            Hall S-474, San Francisco, CA 94158, USA
REFERENCE   3  (bases 1 to 10819)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10819)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-MAY-2018) Cellular and Molecular Pharmacology, University of
            California, San Francisco, 600 16th Street, Genentech Hall S-474,
            San Francisco, CA 94158, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      44..424
                     /label="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     protein_bind    complement(2606..2837)
                     /label="lambda attP"
                     /note="integrase from phage lambda"
     misc_feature    2993..5547
                     /label="Insert F"
                     /note="Insert F"
     CDS             5555..7147
                     /gene="B"
                     /label="Portal protein B"
                     /note="Portal protein B from Escherichia phage lambda.
                     Accession#: P03710"
     rep_origin      8108..8437
                     /note="M13term; M13 origin modified with 3-base mutation to
                     eliminate initation capabilities, resulting in M13
                     terminator"
     misc_feature    8388..8392
                     /label="term-alpha"
                     /note="term-alpha"
     misc_feature    8406..8410
                     /label="term-beta"
                     /note="term-beta"
     misc_feature    8415..8420
                     /label="term-gamma"
                     /note="term-gamma"
     misc_feature    8424..8429
                     /note="term-delta-T3; TGGAAC mutated to TGGTTT"
     primer_bind     complement(8453..8469)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    8477..8493
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8501..8531)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8546..8567)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8855..9443)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9617..10474)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10475..10579)
                     /label="AmpR promoter"