pScaf-7560.1 vector (V003434)

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LOCUS       40924_38898       10315 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Phagemid vector pScaf-7560.1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10315)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM.
  TITLE     Construction of a novel phagemid to produce custom DNA origami 
            scaffolds
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10315)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2018) Cellular and Molecular Pharmacology, 
            University of California, San Francisco, 600 16th Street, Genentech 
            Hall S-474, San Francisco, CA 94158, USA
REFERENCE   3  (bases 1 to 10315)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10315)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-MAY-2018) Cellular and Molecular Pharmacology, University of 
            California, San Francisco, 600 16th Street, Genentech Hall S-474, 
            San Francisco, CA 94158, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      44..424
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     misc_feature    425..7603
                     /label=p7560.1 insert
                     /note="p7560.1 insert"
     rep_origin      7604..7933
                     /note="M13term; M13 origin modified with 3-base mutation to
                     eliminate initation capabilities, resulting in M13 
                     terminator"
     misc_feature    7884..7888
                     /label=term-alpha
                     /note="term-alpha"
     misc_feature    7902..7906
                     /label=term-beta
                     /note="term-beta"
     misc_feature    7911..7916
                     /label=term-gamma
                     /note="term-gamma"
     misc_feature    7920..7925
                     /note="term-delta-T3; TGGAAC mutated to TGGTTT"
     primer_bind     complement(7949..7965)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7973..7989
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7997..8027)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8042..8063)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8351..8939)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9113..9970)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9971..10075)
                     /label=AmpR promoter