pSBFLPS vector (V003447)

LOCUS       40924_38808        5769 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSBFLPS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Bowden SD, Palani NP, Libourel IG.
  TITLE     Stringent control of FLP recombinase in Escherichia coli
  JOURNAL   J. Microbiol. Methods 133, 52-54 (2016)
  PUBMED    28024983
REFERENCE   2  (bases 1 to 5769)
  AUTHORS   Bowden SD, Palani NP, Libourel IG.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2016) Biotechnology Institute, University of 
            Minnesota, 1500 Gortner Avenue, Saint Paul, MN 55108, USA
REFERENCE   3  (bases 1 to 5769)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5769)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Microbiol. Methods 133, 52-54 (2016)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-DEC-2016) Biotechnology Institute, University of Minnesota, 1500
            Gortner Avenue, Saint Paul, MN 55108, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /label=AmpR promoter
     CDS             132..989
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      996..1035
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1182..1290
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      1841..2063
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             2111..3058
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     CDS             complement(3256..4524)
                     /label=FLP
                     /note="site-specific recombinase"
     RBS             4535..4543
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      complement(4536..4541)
                     /regulatory_class="ribosome_binding_site"
     promoter        complement(4570..4854)
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             4881..5756
                     /label=araC
                     /note="L-arabinose regulatory protein"