pSB4K5-LexA-VHH-Caffeine vector (V003451)

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LOCUS       40924_38783        7056 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSB4K5-LexA-VHH-Caffeine, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Chang HJ, Mayonove P, Zavala A, De Visch A, Minard P, Cohen-Gonsaud 
            M, Jerome B.
  TITLE     A modular receptor platform to expand the sensing repertoire of 
            bacteria
  JOURNAL   ACS Synth Biol (2017) In press
  PUBMED    28946740
REFERENCE   2  (bases 1 to 7056)
  AUTHORS   Chang H-J., Mayonove P, De Visch A, Minard P, Cohen-Gonsaud M, 
            Bonnet J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2017) Biophysics, Centre de Biochimie Structurale 
            de Montpellier, 29 Rue de Navacelles, Montpellier 34090, France
REFERENCE   3  (bases 1 to 7056)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7056)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-OCT-2017) Biophysics, Centre de Biochimie Structurale de 
            Montpellier, 29 Rue de Navacelles, Montpellier 34090, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      complement(89..253)
                     /label=Terminator Benchmarking
                     /note="Terminator Benchmarking"
                     /regulatory_class="terminator"
     CDS             complement(297..1010)
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Nager et al., 2011)"
     regulatory      complement(1011..1022)
                     /label=RBS K143021
                     /note="RBS K143021"
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(1031..1111)
                     /label=PsulA408
                     /note="PsulA408"
                     /regulatory_class="promoter"
     terminator      1157..1184
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    1287..1303
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1310..1326
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      1346..1369
                     /label=RBS benchmarking
                     /note="RBS benchmarking"
                     /regulatory_class="ribosome_binding_site"
     CDS             1370..1621
                     /label=LexA BD
                     /note="DNA-binding domain of E. coli LexA"
     CDS             2036..2065
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     terminator      2163..2234
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2250..2277
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             2518..3597
                     /label=lacI
                     /note="lac repressor"
     terminator      3716..3747
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     CDS             complement(3849..4661)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(5022..5969)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     rep_origin      complement(6017..6239)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(6731..6825)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      6862..6921
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(6981..7010)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"