pSB4K5-I52002 vector (V003452)

LOCUS       40924_38778        4509 bp DNA     circular SYN 18-DEC-2018
DEFINITION  BioBrick cloning vector pSB4K5-I52002, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Shetty RP, Endy D, Knight TF Jr.
  TITLE     Engineering BioBrick vectors from BioBrick parts
  JOURNAL   J Biol Eng 2, 5 (2008)
  PUBMED    18410688
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Shetty RP, Endy D, Knight TF Jr.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2008) Biological Engineering, MIT, 32 Vassar 
            Street Room 32-311, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 4509)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4509)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol Eng 
            2, 5 (2008)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-FEB-2008) Biological Engineering, MIT, 32 Vassar Street Room 
            32-311, Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..21
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     misc_feature    35..46
                     /note="translational stop sequence for BioBrick
                     vector-insert insulation, has stop codons in all 6 reading 
                     frames (BBa_B0042)"
     terminator      57..88
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     primer_bind     complement(116..135)
                     /note="VR reverse sequencing primer annealing site
                     (BBa_G00102)"
     regulatory      136..176
                     /note="derived from ribosomal RNA rrnC operon, reverse 
                     orientation (BBa_B0062); similar to Escherichia coli strain
                     MG1655 accession U00096"
                     /regulatory_class="terminator"
     stem_loop       complement(149..168)
                     /label=derived from ribosomal RNA rrnC operon
                     /note="derived from ribosomal RNA rrnC operon"
     CDS             complement(190..1002)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(1363..2310)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     regulatory      complement(2312..2317)
                     /gene="rep101"
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(2314..2326)
                     /gene="rep101"
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(2341..2346)
                     /gene="rep101"
                     /regulatory_class="minus_10_signal"
     rep_origin      complement(2358..2580)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     misc_feature    complement(2692..3059)
                     /label=par region
                     /note="par region"
     terminator      complement(3072..3166)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      3203..3262
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     3275..3294
                     /note="VF2 forward sequencing primer annealing site 
                     (BBa_G00100)"
     terminator      complement(3322..3351)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     misc_feature    3373..3384
                     /note="translational stop sequence for BioBrick
                     vector-insert insulation, has stop codons in all 6 reading 
                     frames (BBa_B0042)"
     misc_feature    3398..3419
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     regulatory      3460..3465
                     /gene="ccdB"
                     /regulatory_class="minus_35_signal"
     regulatory      3483..3488
                     /gene="ccdB"
                     /regulatory_class="minus_10_signal"
     CDS             3527..3829
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     rep_origin      3862..4450
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"